Anti-gamma-Tubulin Purified

Anti-gamma-Tubulin Purified
Regulatory status
RUO
Antigen
gamma-Tubulin
Clone
TU-32
Format
Purified
Reactivity
Protozoa, Plants, Animals
Application
WB, ICC
Variant
0.1 mg
11-543-C100
In stock

0.025 mg
11-543-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody TU-32 recognizes C-terminus (amino acids 434-449 in human) of gamma-tubulin, a 48 kDa structural constituent of cytoskeleton and microtubule organizing center (MTOC). The epitope was located in the aminoacid sequence PDYISW (aa441-446 in human), which is identical for gamma-tubulin 1 and gamma-tubulin 2.
Application
WB, ICC
Reactivity
Protozoa, Plants, Animals
Immunogen
human gamma-tubulin peptide EYHAATRPDYISWGTQ, amino acids 434-449
Other names
TUBG
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-543 WB
Western blotting analysis of gamma-tubulin in porcine brain lysate by antibody TU-32.
11-543 WB
Western blotting analysis of differential reactivity of monoclonal antibodies to γ-tubulin with human γ-tubulin isotypes. (A) Immunoblots of total cell lysates from SH-SY5Y cells, expressing TagRFP-tagged human γ-tubulin 1 (γ-Tb1) or γ-tubulin 2 (γ-Tb2), probed with Abs to γ-tubulin (TU-30, TU-32), TagRFP (RFP) and GAPDH. In control samples, only secondary anti-mouse Ab was applied. (B) Immunoblots of immobilized GST-tagged human C-terminal regions (a.a. 362-451) of γ-Tb1 or γ-Tb2 probed with Abs to γ-tubulin (TU-30, TU-32) and GST. In control samples, only secondary anti-mouse Ab was applied.

General references:

Wiese C, Zheng Y: Microtubule nucleation: gamma-tubulin and beyond. J Cell Sci. 2006 Oct 15;119(Pt 20):4143-53.
PubMed
Haren L, Remy MH, Bazin I, Callebaut I, Wright M, Merdes A. NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly. J Cell Biol. 2006 Feb 13;172(4):505-15.
PubMed
Sulimenko V, Dráberová E, Sulimenko T, Macurek L, Richterová V, Dráber P, Dráber P: Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases. J Immunol. 2006 Jun 15;176(12):7243-53.
PubMed
Wiese C, Zheng Y: Microtubule nucleation: gamma-tubulin and beyond. J Cell Sci. 2006 Oct 15;119(Pt 20):4143-53.
PubMed
Draberova L, Draberova E, Surviladze Z, Draber P, Draber P: Protein tyrosine kinase p53/p56 (lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells. Int Immunol. 1999 Nov;11(11):1829-39.
PubMed
Haren L, Remy MH, Bazin I, Callebaut I, Wright M, Merdes A. NEDD1-dependent recruitment of the gamma-tubulin ring complex to the centrosome is necessary for centriole duplication and spindle assembly. J Cell Biol. 2006 Feb 13;172(4):505-15.
PubMed

Product specific references:

Dráberová E, Sulimenko V, Vinopal S, Sulimenko T, Sládková V, D'Agostino L, Sobol M, Hozák P, Křen L, Katsetos CD, Dráber P: Differential expression of human γ-tubulin isotypes during neuronal development and oxidative stress points to a γ-tubulin-2 prosurvival function. FASEB J. 2017 May;31(5):1828-1846.
PubMed
Katsetos CD, Dráberová E, Smejkalová B, Reddy G, Bertrand L, de Chadarévian JP, Legido A, Nissanov J, Baas PW, Dráber P: Class III beta-tubulin and gamma-tubulin are co-expressed and form complexes in human glioblastoma cells. Neurochem Res. 2007 Aug;32(8):1387-98.
PubMed
Sulimenko V, Dráberová E, Sulimenko T, Macurek L, Richterová V, Dráber P, Dráber P: Regulation of microtubule formation in activated mast cells by complexes of gamma-tubulin with Fyn and Syk kinases. J Immunol. 2006 Jun 15;176(12):7243-53.
PubMed
Katsetos CD, Reddy G, Dráberová E, Smejkalová B, Del Valle L, Ashraf Q, Tadevosyan A, Yelin K, Maraziotis T, Mishra OP, Mörk S, Legido A, Nissanov J, Baas PW, de Chadarévian JP, Dráber P: Altered cellular distribution and subcellular sorting of gamma-tubulin in diffuse astrocytic gliomas and human glioblastoma cell lines. J Neuropathol Exp Neurol. 2006 May;65(5):465-77.
PubMed
Libusová L, Sulimenko T, Sulimenko V, Hozák P, Dráber P: gamma-Tubulin in Leishmania: cell cycle-dependent changes in subcellular localization and heterogeneity of its isoforms. Exp Cell Res. 2004 May 1;295(2):375-86.
PubMed
Sulimenko V, Sulimenko T, Poznanovic S, Nechiporuk-Zloy V, Böhm KJ, Macurek L, Unger E, Dráber P: Association of brain gamma-tubulins with alpha beta-tubulin dimers. Biochem J. 2002 Aug 1;365(Pt 3):889-95.
PubMed
Dráberová E, Macůrek L, Richterová V, Böhm KJ, Dráber P: Monoclonal antibody KN-01 against the heavy chain of kinesin. Folia Biol (Praha). 2002;48(2):77-9.
Linhartová I, Novotná B, Sulimenko V, Dráberová E, Dráber P: Gamma-tubulin in chicken erythrocytes: changes in localization during cell differentiation and characterization of cytoplasmic complexes. Dev Dyn. 2002 Mar;223(2):229-40.
PubMed
Nohýnková E., Dráber P., Reishig J., Kulda J: Cell-cycle dependent localization of gamma-tubulin in early branching eukaryote, a diplomonad flagellate Giardia intestinalis. Eur. J. Cell Biol. 78: 438-445, 2000.
Binarová P, Cenklová V, Hause B, Kubátová E, Lysák M, Dolezel J, Bögre L, Dráber P: Nuclear gamma-tubulin during acentriolar plant mitosis. Plant Cell. 2000 Mar;12(3):433-42.
PubMed
Dráberová L, Dráberová E, Surviladze Z, Dráber P, Dráber P. Protein tyrosine kinase p53/p56(lyn) forms complexes with gamma-tubulin in rat basophilic leukemia cells. Int Immunol. 1999 Nov;11(11):1829-39.
PubMed
Binarová P., Doležel J., Hause B., Dráber P: Association of g-tubulin with kinetochore/centromeric region of plant chromosomes. Plant J. 14: 751-757, 1998.
PubMed
Nováková M, Dráberová E, Schürmann W, Czihak G, Viklický V, Dr-aber P: gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies. Cell Motil Cytoskeleton. 1996;33(1):38-51.
PubMed
Variant
0.1 mg
11-543-C100
In stock

0.025 mg
11-543-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock