Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-gamma-Tubulin GCP2 Purified

Anti-gamma-Tubulin GCP2 Purified

Regulatory status

RUO

Antigen

gamma-Tubulin GCP2

Clone

GCP2-01

Format

Purified

Reactivity

Rat, Mouse, Pig, Human

Application

IP, WB, IHC(P), ICC

Variant

0.1 mg

11-695-C100

In stock

165.00 USD

0.025 mg

11-695-C025

Delivery 1 week

82.50 USD

Variant

0.1 mg

11-695-C100

In stock

165.00 USD

0.025 mg

11-695-C025

Delivery 1 week

82.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody GCP2-01 recognizes gamma-tubulin complex component 2 (GCP2), a 95-103 kDa intracellular protein. It recognizes also phosphorylated GCP2.

Application

IP, WB, IHC(P), ICC

Application details

Western blotting: Recommended dilution: 1-2 μg/ml, reducing conditions.

Reactivity

Rat, Mouse, Pig, Human

Immunogen

GST-fusion protein containing amino acids 2-194 of mouse GCP2

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

GCP-2, GTCC2, GTC2

Antigen description

Gamma tubulin complex component 2 (GCP2) is 95-103 kDa protein which associates with two molecules of gamma tubulin and one molecule of gamma tubulin complex component 3 (GCP3), to form the gamma tubulin small complexes (gammaTuSCs). These complexes interact with GCP4, 5, and 6 to form the gamma tubulin ring complexes (gammaTuRCs), which are embedded in the microtubule organizing center matrix where they nucleate microtubules. The gammaTuRCs are also involved in the regulation of microtubule plus and minus end dynamics. Components of gamma tubulin complexes are highly conservative in eukaryotes.

Entrez Gene ID 10844

UniProt ID Q9BSJ2

Western blotting analysis of human gamma-tubulin complex component 2 (GCP2) using mouse monoclonal antibody GCP2-01 on lysates of various cell lines under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-GCP2 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for GCP2 at approximately 95 kDa.

General references:

Bouissou A, Vérollet C, Sousa A, Sampaio P, Wright M, Sunkel CE, Merdes A, Raynaud-Messina B. {gamma}-Tubulin ring complexes regulate microtubule plus end dynamics. J Cell Biol. 2009 Nov 2;187(3):327-34.
PubMed

Product specific references:

Hořejší B, Vinopal S, Sládková V, Dráberová E, Sulimenko V, Sulimenko T, Vosecká V, Philimonenko A, Hozák P, Katsetos CD, Dráber P: Nuclear γ-tubulin associates with nucleoli and interacts with tumor suppressor protein C53. J Cell Physiol. 2012 Jan;227(1):367-82.
PubMed

Dráberová E, D'Agostino L, Caracciolo V, Sládková V, Sulimenko T, Sulimenko V, Sobol M, Maounis NF, Tzelepis E, Mahera E, Křen L, Legido A, Giordano A, Mörk S, Hozák P, Dráber P, Katsetos CD: Overexpression and Nucleolar Localization of γ-Tubulin Small Complex Proteins GCP2 and GCP3 in Glioblastoma. J Neuropathol Exp Neurol. 2015 Jul;74(7):723-42.
PubMed

Klebanovych A, Sládková V, Sulimenko T, Vosecká V, Čapek M, Dráberová E, Dráber P, Sulimenko V: Regulation of microtubule nucleation in mouse bone marrow-derived mast cells by protein tyrosine phosphatase SHP-1. Cells 2019 Apr 11;8(4):345.
PubMed

Klebanovych A, Vinopal S, Dráberová E, Sládková V, Sulimenko T, Sulimenko V, Vosecká V, Macůrek L, Legido A, Dráber P: C53 interacting with UFM1-protein ligase 1 regulates microtubule nucleation in response to ER stress. Cells 2022 Feb 5;11(3):555.
PubMed

Nejedlá M, Klebanovych A, Sulimenko V, Sulimenko T, Dráberová E, Dráber P, Karlsson R: The actin regulator profilin 1 is functionally associated with the mammalian centrosome. Life Sci Alliance 2020, Nov 12;4(1):e202000655.
PubMed

Ivanova EL, Gilet JG, Sulimenko V, Duchon A, Rudolf G, Runge K, Collins SC, Asselin L, Broix L, Drouot N, Tilly P, Nusbaum P, Vincent A, Magnant W, Skory V, Birling MC, Pavlovic G, Godin JD, Yelcin B, Hérault Y, Dráber P, Chelly J, Hinckelmann MV: TUBG1 missense variants underlying cortical malformations disrupt neuronal locomotion and microtubule dynamics but not neurogenesis. Nat Commun. 2019 May 13;10(1):2129.
PubMed