Anti-Drebrin Purified

Anti-Drebrin Purified
Regulatory status
RUO
Antigen
Drebrin
Clone
DBN-N-03
Format
Purified
Reactivity
Human
Variant
0.1 mg
11-694-C100
In stock
180.00 USD

0.025 mg
11-694-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2b
Specificity
The mouse monoclonal antibody DBN-N-03 recognizes drebrin, an approximately 100-125 kDa intracellular regulator of actin cytoskeleton.
Application details
Western blotting: Positive control: REH, Nalm-6, HEK293 cells.
Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.
Reactivity
Human
Immunogen
Bacterially expressed N-terminal fragment of recombinant human drebrin (aa 1-326)
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
DBN, D0S117E
Antigen description
Drebrin is an actin-binding protein, which is expressed mainly in neurons and plays important role in their morphogenesis. The highest level of its expression is in developing brain. Both in neurons and non-neuronal cells drebrin acts as a key regulator of actin cytoskeleton remodelling, affecting especially intercellular junctions, such as dendritic spines of neurons or the immune synapses of T cells. Decrease of drebrin amount in the brain seems to be associated with Alzheimer's disease and Down syndrome, and in case of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) lower drebrin expression correlates with higher risk of relapse.
Entrez Gene ID 1627
UniProt ID Q16643
11-694 WB
Western blotting analysis of drebrin expression in REH, Ramos, and Nalm-6 cell lysate using mouse monoclonal antibody DBN-N-03.
11-694 MC
Mass cytometry of brain tumor cells with anti-drebrin (DBN-N-03) 152Sm. Gated on singlets.

General references:

Pérez-Martínez M, Gordón-Alonso M, Cabrero JR, Barrero-Villar M, Rey M, Mittelbrunn M, Lamana A, Morlino G, Calabia C, Yamazaki H, Shirao T, Vázquez J, González-Amaro R, Veiga E, Sánchez-Madrid F: F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. J Cell Sci. 2010 Apr 1;123(Pt 7):1160-70.
PubMed
Hayashi K, Ishikawa R, Kawai-Hirai R, Takagi T, Taketomi A, Shirao T: Domain analysis of the actin-binding and actin-remodeling activities of drebrin. Exp Cell Res. 1999 Dec 15;253(2):673-80.
PubMed
Hayashi K, Ishikawa R, Ye LH, He XL, Takata K, Kohama K, Shirao T: Modulatory role of drebrin on the cytoskeleton within dendritic spines in the rat cerebral cortex. J Neurosci. 1996 Nov 15;16(22):7161-70.
PubMed
Sasaki Y, Hayashi K, Shirao T, Ishikawa R, Kohama K: Inhibition by drebrin of the actin-bundling activity of brain fascin, a protein localized in filopodia of growth cones. J Neurochem. 1996 Mar;66(3):980-8.
PubMed
Ishikawa R, Hayashi K, Shirao T, Xue Y, Takagi T, Sasaki Y, Kohama K: Drebrin, a development-associated brain protein from rat embryo, causes the dissociation of tropomyosin from actin filaments. J Biol Chem. 1994 Nov 25;269(47):29928-33.
PubMed

Product specific references:

Vaskova M, Kovac M, Volna P, Angelisova P, Mejstrikova E, Zuna J, Brdicka T, Hrusak O: High expression of cytoskeletal protein drebrin in TEL/AML1pos B-cell precursor acute lymphoblastic leukemia identified by a novel monoclonal antibody. Leuk Res. 2011 Aug;35(8):1111-3. Epub 2011 Apr 16.
PubMed
Variant
0.1 mg
11-694-C100
In stock
180.00 USD

0.025 mg
11-694-C025
Delivery 1 week
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD

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