Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Drebrin Purified

Anti-Drebrin Purified

Regulatory status

RUO

Antigen

Drebrin

Clone

DBN-N-03

Format

Purified

Reactivity

Human

Application

MC (CyTOF), WB, FC (QC tested)

Variant

0.1 mg

11-694-C100

In stock

198.00 USD

0.025 mg

11-694-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-694-C100

In stock

198.00 USD

0.025 mg

11-694-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b

Specificity

The mouse monoclonal antibody DBN-N-03 recognizes drebrin, an approximately 100-125 kDa intracellular regulator of actin cytoskeleton.

Application

MC (CyTOF), WB, FC (QC tested)

Application details

Western blotting: Positive control: REH, Nalm-6, HEK293 cells.
Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

Reactivity

Human

Immunogen

Bacterially expressed N-terminal fragment of recombinant human drebrin (aa 1-326)

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

DBN, D0S117E

Antigen description

Drebrin is an actin-binding protein, which is expressed mainly in neurons and plays important role in their morphogenesis. The highest level of its expression is in developing brain. Both in neurons and non-neuronal cells drebrin acts as a key regulator of actin cytoskeleton remodelling, affecting especially intercellular junctions, such as dendritic spines of neurons or the immune synapses of T cells. Decrease of drebrin amount in the brain seems to be associated with Alzheimer's disease and Down syndrome, and in case of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) lower drebrin expression correlates with higher risk of relapse.

Entrez Gene ID 1627

UniProt ID Q16643

Western blotting analysis of drebrin expression in REH, Ramos, and Nalm-6 cell lysate using mouse monoclonal antibody DBN-N-03.

Mass cytometry of brain tumor cells with anti-drebrin (DBN-N-03) 152Sm. Gated on singlets.

General references:

Pérez-Martínez M, Gordón-Alonso M, Cabrero JR, Barrero-Villar M, Rey M, Mittelbrunn M, Lamana A, Morlino G, Calabia C, Yamazaki H, Shirao T, Vázquez J, González-Amaro R, Veiga E, Sánchez-Madrid F: F-actin-binding protein drebrin regulates CXCR4 recruitment to the immune synapse. J Cell Sci. 2010 Apr 1;123(Pt 7):1160-70.
PubMed

Hayashi K, Ishikawa R, Kawai-Hirai R, Takagi T, Taketomi A, Shirao T: Domain analysis of the actin-binding and actin-remodeling activities of drebrin. Exp Cell Res. 1999 Dec 15;253(2):673-80.
PubMed

Hayashi K, Ishikawa R, Ye LH, He XL, Takata K, Kohama K, Shirao T: Modulatory role of drebrin on the cytoskeleton within dendritic spines in the rat cerebral cortex. J Neurosci. 1996 Nov 15;16(22):7161-70.
PubMed

Sasaki Y, Hayashi K, Shirao T, Ishikawa R, Kohama K: Inhibition by drebrin of the actin-bundling activity of brain fascin, a protein localized in filopodia of growth cones. J Neurochem. 1996 Mar;66(3):980-8.
PubMed

Ishikawa R, Hayashi K, Shirao T, Xue Y, Takagi T, Sasaki Y, Kohama K: Drebrin, a development-associated brain protein from rat embryo, causes the dissociation of tropomyosin from actin filaments. J Biol Chem. 1994 Nov 25;269(47):29928-33.
PubMed

Product specific references:

Vaskova M, Kovac M, Volna P, Angelisova P, Mejstrikova E, Zuna J, Brdicka T, Hrusak O: High expression of cytoskeletal protein drebrin in TEL/AML1pos B-cell precursor acute lymphoblastic leukemia identified by a novel monoclonal antibody. Leuk Res. 2011 Aug;35(8):1111-3. Epub 2011 Apr 16.
PubMed