Anti-Cytokeratins Purified

Anti-Cytokeratins Purified
Regulatory status
RUO
Antigen
Cytokeratins
Clone
C-11
Format
Purified
Reactivity
Mammalian
Variant
0.1 mg
11-108-C100
In stock
143.00 USD

0.025 mg
11-108-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-108-C100
In stock
143.00 USD

0.025 mg
11-108-C025
Delivery 1 week
71.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.
Application details
Flow cytometry: Recommended dilution: 1 μg/ml. Intracellular staining.
Immunohistochemistry: Recommended dilution: 2-8 μg/ml.
Western blotting: Recommended dilution: 1-2 μg/ml.
Reactivity
Mammalian
Immunogen
Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
cytokeratin, CYK, CK, KRT
Antigen description
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
11-108_ICC
Immunocytochemistry staining of cytokeratins in Hep-2 cells using pan-cytokeratin antibody C-11 (11-108-C100, diluted 1:400), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green).
11-108_FC_Histogram
Separation of MCF-7 cells (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 μg/ml, GAM APC).
11-108_FC_Profil Značení
Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratins (C-11) purified antibody (concentration in sample 3 μg/ml, GAM APC).
11-108_IHC
Immunohistochemistry staining of cytokeratin on paraffin-embedded sections of guinea pig breast carcinoma using anti-cytokeratin antibody (C-11).
11-108_WB
Western blotting analysis of human cytokeratins using mouse monoclonal antibody C-11 on lysates of HeLa cell line and MOLT-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody anti-cytokeratins followed by IRDye800-conjugated anti-mouse secondary antibody. Specific bands were detected for cytokeratins at approximately 45-55 kDa.
11-108_WB_react
Anti-Hu Cytokeratins Purified (clone C-11) reactivity pattern in WB application.
The reactivity of C-11 antibody was assessed by comparing binding signals in a panel of cytokeratin-positive and cytokeratin-negative human cell lines. Western blotting analysis was performed on whole cell extracts (hot 1% SDS lysis buffer) of HeLa, MCF-7, Jurkat, and Molt-4 cell lines, mixed and heated (100°C, 5 min) with reducing (2-mercaptoethanol) SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody C-11 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). For multiplex fluorescent Western blot detection, mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml) was used as the loading control (red).

Cytokeratins were detected at ~38-55 kDa in the respective cell lines.

Product specific references:

Hamakawa H, Sumida T, Tanioka H, Sogawa K, Yamada T: Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis. Res Commun Mol Pathol Pharmacol. 1998 Aug;101(2):115-26.
PubMed
Broekema M, Harmsen MC, Koerts JA, Petersen AH, van Luyn MJ, Navis G, Popa ER: Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats. Kidney Int. 2005 Dec;68(6):2572-81.
PubMed
Vojtesek B, Stasková Z, Nenutil R, Lauerová L, Kovarík J, Rejthar A, Bártková J, Bártek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82.
PubMed
Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5.
PubMed
Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24.
PubMed
Variant
0.1 mg
11-108-C100
In stock
143.00 USD

0.025 mg
11-108-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-108-C100
In stock
143.00 USD

0.025 mg
11-108-C025
Delivery 1 week
71.50 USD

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