Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Cytokeratins PE

Anti-Cytokeratins PE

Regulatory status

RUO

Antigen

Cytokeratins

Clone

C-11

Format

Reactivity

Mammalian

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1P-108-C100

In stock

275.00 USD

0.025 mg

1P-108-C025

Delivery 1 week

137.50 USD

Variant

0.1 mg

1P-108-C100

In stock

275.00 USD

0.025 mg

1P-108-C025

Delivery 1 week

137.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1 μg/ml. Intracellular staining.

Reactivity

Mammalian

Immunogen

Keratin-enriched preparation from human epidermoid carcinoma cell line A431.

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

cytokeratin, CYK, CK, KRT

Antigen description

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Separation of HeLa cells from human lymphocytes stained using anti-human Cytokeratin (pan-reactive) (C-11) PE antibody (concentration in sample 5 μg/ml 100 μl of cell suspension, red-filled) in flow cytometry analysis (intracellular staining) of peripheral whole blood spiked with HeLa cells.

Product specific references:

Hamakawa H, Sumida T, Tanioka H, Sogawa K, Yamada T: Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis. Res Commun Mol Pathol Pharmacol. 1998 Aug;101(2):115-26.
PubMed

Broekema M, Harmsen MC, Koerts JA, Petersen AH, van Luyn MJ, Navis G, Popa ER: Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats. Kidney Int. 2005 Dec;68(6):2572-81.
PubMed

Vojtesek B, Stasková Z, Nenutil R, Lauerová L, Kovarík J, Rejthar A, Bártková J, Bártek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82.
PubMed

Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5.
PubMed

Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24.
PubMed