Anti-Cytokeratins FITC

Anti-Cytokeratins FITC
Regulatory status
RUO
Antigen
Cytokeratins
Clone
C-11
Format
FITC
Reactivity
Mammalian
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1F-108-C100
In stock
231.00 USD

0.025 mg
1F-108-C025
Delivery 1 week
115.50 USD
Variant
0.1 mg
1F-108-C100
In stock
231.00 USD

0.025 mg
1F-108-C025
Delivery 1 week
115.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody C-11 reacts with cytokeratin peptides 4, 5, 6, 8, 10, 13, 18. Cytokeratins are members of intermediate filaments subfamily intracellular proteins represented in epithelial tissues.
Application
Application details
Flow cytometry: Recommended dilution: 1 μg/ml. Intracellular staining.
Reactivity
Mammalian
Immunogen
Keratin-enriched preparation from human epidermoid carcinoma cell line A431.
Concentration
1 mg/ml
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
cytokeratin, CYK, CK, KRT
Antigen description
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
1F-108_FC_Profil Značení
Flow cytometry surface staining pattern of human peripheral whole blood spiked with HeLa cells stained using anti-Cytokeratins (C-11) FITC antibody (concentration in sample 1.5 μg/ml).
1F-108_FC_Histogram
Anti-Hu Cytokeratins FITC antibody (clone C-11) specificity verification by flow cytometry

Anti-Cytokeratins (C-11) FITC antibody (concentration in sample 1.5 μg/ml, red-filled histogram) binds specifically to intracellular cytokeratins in permeabilized HeLa cells (upper panel), but does not stain permeabilized TF-1 cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control FITC antibody (MOPC-21) under same conditions (concentration in sample 1.5 μg/ml, black-dashed histogram).

Product specific references:

Hamakawa H, Sumida T, Tanioka H, Sogawa K, Yamada T: Extraction of cytokeratin from the human submandibular gland and its electrophoretic analysis. Res Commun Mol Pathol Pharmacol. 1998 Aug;101(2):115-26.
PubMed
Broekema M, Harmsen MC, Koerts JA, Petersen AH, van Luyn MJ, Navis G, Popa ER: Determinants of tubular bone marrow-derived cell engraftment after renal ischemia/reperfusion in rats. Kidney Int. 2005 Dec;68(6):2572-81.
PubMed
Vojtesek B, Stasková Z, Nenutil R, Lauerová L, Kovarík J, Rejthar A, Bártková J, Bártek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82.
PubMed
Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5.
PubMed
Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24.
PubMed
Variant
0.1 mg
1F-108-C100
In stock
231.00 USD

0.025 mg
1F-108-C025
Delivery 1 week
115.50 USD
Variant
0.1 mg
1F-108-C100
In stock
231.00 USD

0.025 mg
1F-108-C025
Delivery 1 week
115.50 USD

Related products

-134%
CloneMOPC-21
Reg. statusRUO
ApplicationFC, FC-IC
0.1 mg
165.00 USD