Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Cytokeratin 8 Purified

Anti-Cytokeratin 8 Purified

Regulatory status

RUO

Antigen

Cytokeratin 8

Clone

C-43

Format

Purified

Reactivity

Cow, Human, Rabbit, Pig, Sheep

Application

FC-IC, IP, WB, IHC-P, ICC

Variant

0.1 mg

11-105-C100

In stock

143.00 USD

0.025 mg

11-105-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-105-C100

In stock

143.00 USD

0.025 mg

11-105-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody C-43 reacts with cytokeratin 8 (52.5 kDa intracellular antigen). Cytokeratins are members of intermediate filaments subfamily represented in epithelial tissues.

Application

FC-IC, IP, WB, IHC-P, ICC

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: prostate.
Flow cytometry: Recommended dilution: 0.5-4 μg/ml. Intracellular staining.

Reactivity

Cow, Human, Rabbit, Pig, Sheep

Negative species

Mouse, Rat, Xenopus, Hamster, Chicken

Immunogen

Cytoskeleton preparation from HeLa human cervix carcinoma cell line.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

K8, CK8, CYK8, KRT8, K2C8, CARD2

Antigen description

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Entrez Gene ID 3856

UniProt ID Q7L4M3

Immunocytochemistry staining of cytokeratin 8 in Hep-2 cells using mouse monoclonal C-43 (11-105-C100, diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green).

Separation of MCF-7 cells stained using anti-Cytokeratin 8 (C-43) purified antibody (concentration in sample 0.15 μg/ml, GAM APC, red-filled) from MCF-7 cells stained using negative control (MOPC-21) purified antibody (concentration in sample 8 μg/ml, same as Cytokeratin 8 purified concentration, GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Immunohistochemistry staining of human prostate (paraffin sections) using anti-cytokeratin 8(C-43). Commercially tested by LifeSpan BioSciences.

Anti-Hu Cytokeratin 8 Purified (clone C-43) reactivity pattern in WB application.
The reactivity of C-43 antibody was assessed by comparing binding signals in a panel of cytokeratin-positive and cytokeratin-negative human cell lines. Western blotting analysis was performed on whole cell extracts (hot 1% SDS lysis buffer) of HT-29, HeLa, MCF-7, Hep-G2, Huvec, HTR-8/SVneo, Jurkat, and Molt-4 cell lines, mixed and heated (100°C, 5 min) with reducing (2-mercaptoethanol) SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody C-43 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green). For multiplex fluorescent Western blot detection, mouse anti-GAPDH monoclonal antibody FF26A conjugated with DyLight 680 (0.1 µg/ml) was used as the loading control (red).

Cytokeratin 8 was detected at ~52 kDa in the respective cell lines.

Product specific references:

Kovarik J et al.: J Tumor Marker Oncol. 5, 219 (1990).

Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24.
PubMed

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15(11):909-912.
PubMed