Anti-Cytokeratin 8 Purified

Anti-Cytokeratin 8 Purified
Regulatory status
RUO
Antigen
Cytokeratin 8
Clone
C-43
Format
Purified
Reactivity
Cow, Human, Rabbit, Pig, Sheep
Variant
0.1 mg
11-105-C100
In stock
143.00 USD

0.025 mg
11-105-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-105-C100
In stock
143.00 USD

0.025 mg
11-105-C025
Delivery 1 week
71.50 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody C-43 reacts with cytokeratin 8 (52.5 kDa intracellular antigen). Cytokeratins are members of intermediate filaments subfamily represented in epithelial tissues.
Application details
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml; positive tissue: prostate.
Flow cytometry: Recommended dilution: 1-5 μg/ml, intracellular staining.
Reactivity
Cow, Human, Rabbit, Pig, Sheep
Negative species
Mouse, Rat, Xenopus, Hamster, Chicken
Immunogen
Cytoskeleton preparation from HeLa human cervix carcinoma cell line.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
K8, CK8, CYK8, KRT8, K2C8, CARD2
Antigen description
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Entrez Gene ID 3856
UniProt ID Q7L4M3
11-105_ICC
Immunocytochemistry staining of cytokeratin 8 in Hep-2 cells using mouse monoclonal C-43 (11-105-C100, diluted 1:100), detected with GAM IgG-Alexa Fluor®488 (diluted 1:200; green).
11-105_IHC
Immunohistochemistry staining of human prostate (paraffin sections) using anti-cytokeratin 8(C-43). Commercially tested by LifeSpan BioSciences.
11-105 WB
Western blotting detection of cytokeratin 8 in HeLa cell lysate (reducing conditions) by mouse monoclonal antibody C-43.

Product specific references:

Kovarik J et al.: J Tumor Marker Oncol. 5, 219 (1990).
Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24.
PubMed
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15(11):909-912.
PubMed
Variant
0.1 mg
11-105-C100
In stock
143.00 USD

0.025 mg
11-105-C025
Delivery 1 week
71.50 USD
Variant
0.1 mg
11-105-C100
In stock
143.00 USD

0.025 mg
11-105-C025
Delivery 1 week
71.50 USD

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