Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Cytokeratin 8 Purified

Anti-Cytokeratin 8 Purified

Regulatory status

RUO

Antigen

Cytokeratin 8

Clone

C-51

Format

Purified

Reactivity

Pig, Cow, Human, Sheep

Application

IP, WB, IHC-P, ICC

Variant

0.1 mg

11-104-C100

In stock

143.00 USD

0.025 mg

11-104-C025

Delivery 1 week

71.50 USD

Variant

0.1 mg

11-104-C100

In stock

143.00 USD

0.025 mg

11-104-C025

Delivery 1 week

71.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody C-51 recognizes cytokeratin 8 (52.5 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.

Application

IP, WB, IHC-P, ICC

Application details

Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml

Reactivity

Pig, Cow, Human, Sheep

Negative species

Dog, Mouse, Rat, Xenopus, Chicken, Rabbit

Immunogen

Cytoskeleton preparation from HeLa cells.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

K8, CK8, CYK8, KRT8, K2C8, CARD2

Antigen description

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Entrez Gene ID 3856

UniProt ID Q7L4M3

Western blotting detection of cytokeratin 8 in HeLa cell lysate by mouse monoclonal antibody C-51.

Immunocytochemistry staining of cytokeratin 8 in Hep-2 cells using mouse monoclonal antibody C-51 (11-104-C100, diluted 1:200), detected with GAM IgG-Alexa Fluor®488 (diluted 1:100; green).

Product specific references:

Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24.
PubMed