Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Cytokeratin 8 FITC

Anti-Cytokeratin 8 FITC

Regulatory status

RUO

Antigen

Cytokeratin 8

Clone

C-43

Format

FITC

Reactivity

Cow, Human, Rabbit, Pig, Sheep

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1F-105-C100

In stock

198.00 USD

0.025 mg

1F-105-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

1F-105-C100

In stock

198.00 USD

0.025 mg

1F-105-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody C-43 reacts with cytokeratin 8 (52.5 kDa intracellular antigen). Cytokeratins are members of intermediate filaments subfamily represented in epithelial tissues.

Application

FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining.

Reactivity

Cow, Human, Rabbit, Pig, Sheep

Negative species

Mouse, Rat, Xenopus, Hamster, Chicken

Immunogen

Cytoskeleton preparation from HeLa human cervix carcinoma cell line.

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

K8, CK8, CYK8, KRT8, K2C8, CARD2

Antigen description

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Entrez Gene ID 3856

UniProt ID Q7L4M3

Flow cytometry intracellular staining pattern of human peripheral whole blood mixed with A431 cellular suspension stained using anti-Cytokeratin 8 (C-43) FITC antibody (concentration in sample 9 μg/ml).

Separation of A431 cellular suspension (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood mixed with A431 cellular suspension stained using anti-Cytokeratin 8 (C-43) FITC antibody (concentration in sample 9 μg/ml).

Product specific references:

Kovarik J et al.: J Tumor Marker Oncol. 5, 219 (1990).

Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24.
PubMed

Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15(11):909-912.
PubMed