Anti-Cytokeratin 8 FITC

Anti-Cytokeratin 8 FITC
Regulatory status
RUO
Antigen
Cytokeratin 8
Clone
C-43
Format
FITC
Reactivity
Cow, Human, Rabbit, Pig, Sheep
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1F-105-C100
In stock
180.00 USD

0.025 mg
1F-105-C025
In stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody C-43 reacts with cytokeratin 8 (52.5 kDa intracellular antigen). Cytokeratins are members of intermediate filaments subfamily represented in epithelial tissues.
Application
Application details
Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining.
Reactivity
Cow, Human, Rabbit, Pig, Sheep
Negative species
Mouse, Rat, Xenopus, Hamster, Chicken
Immunogen
Cytoskeleton preparation from HeLa human cervix carcinoma cell line.
Concentration
1 mg/ml
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
K8, CK8, CYK8, KRT8, K2C8, CARD2
Antigen description
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Entrez Gene ID 3856
UniProt ID Q7L4M3
1F-105_FC_Profil Značení
Flow cytometry intracellular staining pattern of human peripheral whole blood mixed with A431 cellular suspension stained using anti-Cytokeratin 8 (C-43) FITC antibody (concentration in sample 9 μg/ml).
1F-105_FC_Histogram
Separation of A431 cellular suspension (red-filled) from human leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood mixed with A431 cellular suspension stained using anti-Cytokeratin 8 (C-43) FITC antibody (concentration in sample 9 μg/ml).

Product specific references:

Kovarik J et al.: J Tumor Marker Oncol. 5, 219 (1990).
Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24.
PubMed
Sikorski K, Mehta A, Inngjerdingen M, Thakor F, Kling S, Kalina T, Nyman TA, Stensland ME, Zhou W, de Souza GA, Holden L, Stuchly J, Templin M, Lund-Johansen F: A high-throughput pipeline for validation of antibodies. Nat Methods. 2018 Nov;15(11):909-912.
PubMed
Variant
0.1 mg
1F-105-C100
In stock
180.00 USD

0.025 mg
1F-105-C025
In stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD

0.1 mg
11-261-C100
In stock
90.00 USD

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