Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Cytokeratin 7 Purified

Anti-Cytokeratin 7 Purified

Regulatory status

RUO

Antigen

Cytokeratin 7

Clone

OV-TL 12/30

Format

Purified

Reactivity

Human

Application

IHC-P (reported), IHC-F (reported), WB (verified)

test1 True

Variant

0.1 mg

11-958-C100

In stock

158.40 USD

Variant

0.1 mg

11-958-C100

In stock

158.40 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody OVTL12 recognizes cytokeratin 7, an intermediate filament protein.

Application

IHC-P (reported), IHC-F (reported), WB (verified)

Application details

Western blotting: Recommended dilution: 1 μg/ml, reducing conditions.

Reactivity

Human

Immunogen

OTNII ovarian carcinoma cell line

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

K7, CK7, CYK7, KRT7, SCL, K2C7

Antigen description

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Entrez Gene ID 3855

UniProt ID P08729

Anti-Hu Cytokeratin 7 Purified (clone OV-TL 12/30) reactivity pattern in WB application.
The reactivity of OV-TL 12/30 antibody was assessed by comparing binding signals in a panel of cytokeratin-positive and cytokeratin-negative human cell lines. Western blotting analysis was performed on whole cell extracts (hot 1% SDS lysis buffer) of JAR, JEG3, HTR-8/SVneo, HeLa, MCF-7, Huvec, Hep-G2, Jurkat and Molt-4 cell lines, mixed and heated (100°C, 5 min) with reducing (2-mercaptoethanol) SDS-loading buffer. Samples were resolved using 10% SDS-PAGE gel.

Nitrocellulose membrane blot was probed with mouse IgG1 monoclonal antibody OV-TL 12/30 (1 µg/ml), followed by IRDye 800CW Goat-anti-Mouse IgG (green).

Cytokeratin 7 was detected at ~50 kDa in the respective cell lines.

Product specific references:

van Niekerk CC, Boerman OC, Ramaekers FC, Poels LG: Marker profile of different phases in the transition of normal human ovarian epithelium to ovarian carcinomas. Am J Pathol. 1991 Feb;138(2):455-63.
PubMed

Torenbeek R, Lagendijk JH, Van Diest PJ, Bril H, van de Molengraft FJ, Meijer CJ: Value of a panel of antibodies to identify the primary origin of adenocarcinomas presenting as bladder carcinoma. Histopathology 1998 Jan;32(1):20-7.
PubMed

van Niekerk CC, Vooijs P, Casparie-van Velsen IJ, Poels LG: Differentiation margins of ovarian tumor pathology: First incidences of epithelial ovarian tumors monitored by marker antibodies. Cancer Detect Prev. 1997;21(3):247-57.
PubMed

van Niekerk CC, Jap PH, Ramaekers FC, van de Molengraft F, Poels LG: Immunohistochemical demonstration of keratin 7 in routinely fixed paraffin-embedded human tissues. J Pathol. 1991 Oct;165(2):145-52.
PubMed

Ramaekers F, van Niekerk C, Poels L, Schaafsma E, Huijsmans A, Robben H, Schaart G, Vooijs P: Use of monoclonal antibodies to keratin 7 in the differential diagnosis of adenocarcinomas. Am J Pathol. 1990 Mar;136(3):641-55.
PubMed