Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Cytokeratin 7/17 Purified

Anti-Cytokeratin 7/17 Purified

Anti-Cytokeratin 7/17 Purified
Regulatory status
RUO
Antigen
Cytokeratin 7/17
Clone
C-46
Format
Purified
Reactivity
Pig, Human, Cow
Application
IP, WB, IHC(P), ICC
Variant
0.1 mg
11-109-C100
In stock
130.00 USD
0.025 mg
11-109-C025
Delivery 1 week
65.00 USD
Variant
0.1 mg
11-109-C100
In stock
130.00 USD
0.025 mg
11-109-C025
Delivery 1 week
65.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody C-46 reacts with cytokeratin peptides 7 and 17 (54 and 46 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins represented in epithelial tissues.
Application
IP, WB, IHC(P), ICC
Application details
Immunohistochemistry (paraffin sections): Recommended dilution: 10 μg/ml, pretreatment: 0,1% pepsin, 30 min, 37°C, incubation 4°C overnight, chromogen DAB; positive tissue: pancreas.
Western blotting: Recommended dilution: 1-2 μg/ml; positive control: HeLa cells.
Reactivity
Pig, Human, Cow
Negative species
Rabbit, Mouse, Rat
Immunogen
Cytoskeleton preparation of HeLa human cervix carcinoma cell line.
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
K7, K17, CK7, CD17, CK17, CYK7, CYK17, KRT7, KRT17, SCL, K2C7
Antigen description
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
11-109_IHC
Immunohistochemistry staining of pancreas (paraffin-embedded sections) with anti-Cytokeratin 7+17 (C-46).
11-109_IHC_2
Immunohistochemistry staining of human breast (paraffin-embedded sections) with anti-cytokeratin 7+17 (C-46). Commercially tested by LifeSpan BioSciences.
11-109_WB
Western blotting analysis of human cytokeratin 7+17 using mouse monoclonal antibody C-46 on lysates of HeLa cell line and Jurkat cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-cytokeratin 7+17 monoclonal antibody C-46 followed by IRDye800-conjugated anti-mouse IgG1 secondary antibody. A specific band was detected for cytokeratin 17 at approximately 46 kDa and for cytokeratin 7 at approximately 54 kDa.

Product specific references:

Vojtesek B, Staskova Z, Nenutil R, Bartkova J, Kovarik J, Rejthar A, Bartek J: A panel of monoclonal antibodies to keratin no. 7: characterization and value in tumor diagnosis. Neoplasma. 1990;37(3):333-42.
PubMed
Variant
0.1 mg
11-109-C100
In stock
130.00 USD
0.025 mg
11-109-C025
Delivery 1 week
65.00 USD
Variant
0.1 mg
11-109-C100
In stock
130.00 USD
0.025 mg
11-109-C025
Delivery 1 week
65.00 USD
Contact distributor
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