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Exbio
—
Research products
—
Antibodies
—
Cytoskeleton and ECM
—
Anti-Cytokeratin 19 FITC
Anti-Cytokeratin 19 FITC
Regulatory status
RUO
Antigen
Cytokeratin 19
Clone
A53-B/A2
Format
FITC
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
0.1 mg
1F-120-C100
In stock
231.00 USD
0.025 mg
1F-120-C025
Delivery 1 week
115.50 USD
Variant
0.1 mg
1F-120-C100
In stock
231.00 USD
0.025 mg
1F-120-C025
Delivery 1 week
115.50 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Reactivity
Human
Immunogen
MCF-7 human breast adenocarcinoma cell line
Concentration
1 mg/ml
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
K19, CK19, CYK19, KRT19, K1CS
Antigen description
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Entrez Gene ID
3880
UniProt ID
P08727
Separation of MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) FITC antibody (concentration in sample 3 μg/ml, red-filled) from MCF-7 cells stained using mouse IgG2b isotype control (MPC-11) FITC antibody (concentration in sample 3 μg/ml, same as anti-Cytokeratin 19 FITC antibody concentration, black-dashed) in flow cytometry analysis (surface staining).
Product specific references:
Karsten U, Papsdorf G, Roloff G, Stolley P, Abel H, Walther I, Weiss H: Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion. Eur J Cancer Clin Oncol. 1985 Jun;21(6):733-40.
PubMed
Kasper M, Moll R, Stosiek P, Karsten U: Patterns of cytokeratin and vimentin expression in the human eye. Histochemistry. 1988;89(4):369-77.
PubMed
Bártek J, Bártková J, Taylor-Papadimitriou J, Rejthar A, Kovarík J, Lukás Z, Vojtĕsek B: Differential expression of keratin 19 in normal human epithelial tissues revealed by monospecific monoclonal antibodies. Histochem J. 1986 Oct;18(10):565-75.
PubMed
Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
1F-120-C100
In stock
231.00 USD
0.025 mg
1F-120-C025
Delivery 1 week
115.50 USD
Variant
0.1 mg
1F-120-C100
In stock
231.00 USD
0.025 mg
1F-120-C025
Delivery 1 week
115.50 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
Related products
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Clone
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Reg. status
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Application
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0.1 mg
165.00 USD
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