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Exbio
—
Research products
—
Antibodies
—
Cytoskeleton and ECM
—
Anti-Cytokeratin 19 FITC
Anti-Cytokeratin 19 FITC
Regulatory status
RUO
Antigen
Cytokeratin 19
Clone
A53-B/A2
Format
FITC
Reactivity
Human
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
0.1 mg
1F-120-C100
In stock
210.00 USD
0.025 mg
1F-120-C025
Delivery 1 week
105.00 USD
Variant
0.1 mg
1F-120-C100
In stock
210.00 USD
0.025 mg
1F-120-C025
Delivery 1 week
105.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Reactivity
Human
Immunogen
MCF-7 human breast adenocarcinoma cell line
Concentration
1 mg/ml
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
K19, CK19, CYK19, KRT19, K1CS
Antigen description
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Entrez Gene ID
3880
UniProt ID
P08727
Separation of MCF-7 human breast adenocarcinoma cell line (red) from human peripheral whole blood (blue) in flow cytometry analysis (intracellular staining) stained using anti-human cytokeratin 19 (A53-B/A2) FITC antibody (concentration in sample 9 µg/ml).
Product specific references:
Karsten U, Papsdorf G, Roloff G, Stolley P, Abel H, Walther I, Weiss H: Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion. Eur J Cancer Clin Oncol. 1985 Jun;21(6):733-40.
PubMed
Kasper M, Moll R, Stosiek P, Karsten U: Patterns of cytokeratin and vimentin expression in the human eye. Histochemistry. 1988;89(4):369-77.
PubMed
Bártek J, Bártková J, Taylor-Papadimitriou J, Rejthar A, Kovarík J, Lukás Z, Vojtĕsek B: Differential expression of keratin 19 in normal human epithelial tissues revealed by monospecific monoclonal antibodies. Histochem J. 1986 Oct;18(10):565-75.
PubMed
Variant
0.1 mg
1F-120-C100
In stock
210.00 USD
0.025 mg
1F-120-C025
Delivery 1 week
105.00 USD
Variant
0.1 mg
1F-120-C100
In stock
210.00 USD
0.025 mg
1F-120-C025
Delivery 1 week
105.00 USD
Contact distributor
Datasheet download
SDS download
CS
EN
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Technical support for this product
Related products
1F-724-C100
Mouse IgG2a Isotype Control FITC
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Clone
MOPC-173
Reg. status
RUO
Application
FC, FC(IC)
0.1 mg
150.00 USD
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