Anti-Cytokeratin 19 Alexa Fluor® 488

Anti-Cytokeratin 19 Alexa Fluor<sup>®</sup> 488
Regulatory status
RUO
Antigen
Cytokeratin 19
Clone
A53-B/A2
Format
Alexa Fluor® 488
Reactivity
Human
Excitation laser
blue (488 nm)
Variant
0.1 mg
A4-120-C100
In stock
308.00 USD

0.025 mg
A4-120-C025
Delivery 1 week
154.00 USD
Variant
0.1 mg
A4-120-C100
In stock
308.00 USD

0.025 mg
A4-120-C025
Delivery 1 week
154.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a
Specificity
The mouse monoclonal antibody A53-B/A2 recognizes the rod domain of cytokeratin 19 (40 kDa), an intracellular antigen constituting intermmediate cytoskeleton filaments. Cytokeratin 19 is not expressed in hepatocytes; it is often co-expressed with cytokeratin 7.
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Reactivity
Human
Immunogen
MCF-7 human breast adenocarcinoma cell line
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with Alexa Fluor® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
K19, CK19, CYK19, KRT19, K1CS
Antigen description
Cytokeratins are a subfamily of intermediate filaments and characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Entrez Gene ID 3880
UniProt ID P08727
A4-120_FC_Profil Značení
Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) Alexa Fluor® 488 antibody (concentration in sample 5 μg/ml).
A4-120_FC_Histogram
Separation of human MCF-7 cells (red-filled) from leukocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) Alexa Fluor® 488 antibody (concentration in sample 5 μg/ml).

Product specific references:

Karsten U, Papsdorf G, Roloff G, Stolley P, Abel H, Walther I, Weiss H: Monoclonal anti-cytokeratin antibody from a hybridoma clone generated by electrofusion. Eur J Cancer Clin Oncol. 1985 Jun;21(6):733-40.
PubMed
Kasper M, Moll R, Stosiek P, Karsten U: Patterns of cytokeratin and vimentin expression in the human eye. Histochemistry. 1988;89(4):369-77.
PubMed
Bártek J, Bártková J, Taylor-Papadimitriou J, Rejthar A, Kovarík J, Lukás Z, Vojtĕsek B: Differential expression of keratin 19 in normal human epithelial tissues revealed by monospecific monoclonal antibodies. Histochem J. 1986 Oct;18(10):565-75.
PubMed
Quardokus EM, Saunders DC, McDonough E, Hickey JW, Werlein C, Surrette C, Rajbhandari P, Martinez Casals A, Tian H, Lowery L, Neumann EK, Björklund F, Neelakantan TV, Croteau J, Wiblin AE, Fisher J, Livengood AJ, Dowell KG, Silverstein JC, Spraggins JM, Pryhuber GS, Deutsch G, Ginty F, Nolan GP, Melov S, Jonigk D, Caldwell MA, Vlachos IS, Mueller W, Gehlenborg N, Stockwell BR, Lundberg E, Snyder MP, Germain RN, Camarillo JM, Kelleher NL, Börner K, Radtke AJ: Organ mapping antibody panels: a community resource for standardized multiplexed tissue imaging. Nat Methods 2023 Aug;20(8):1174-1178. doi: 10.1038/s41592-023-01846-7.
PubMed
Variant
0.1 mg
A4-120-C100
In stock
308.00 USD

0.025 mg
A4-120-C025
Delivery 1 week
154.00 USD
Variant
0.1 mg
A4-120-C100
In stock
308.00 USD

0.025 mg
A4-120-C025
Delivery 1 week
154.00 USD

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