Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Cytokeratin 18 FITC

Anti-Cytokeratin 18 FITC

Regulatory status

RUO

Antigen

Cytokeratin 18

Clone

C-04

Format

FITC

Reactivity

Mammalian

Application

FC-IC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1F-106-C100

In stock

237.60 USD

0.025 mg

1F-106-C025

Delivery 1 week

118.80 USD

Variant

0.1 mg

1F-106-C100

In stock

237.60 USD

0.025 mg

1F-106-C025

Delivery 1 week

118.80 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody C-04 reacts with cytokeratin 18 (45 kDa intracellular antigen), a member of intermediate filaments subfamily represented in epithelial tissues.

Application

FC-IC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining.

Reactivity

Mammalian

Immunogen

Cytoskeleton preparation of epidermal carcinoma cell line A431.

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

K18, CK18, CYK18, KRT18

Antigen description

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).

Entrez Gene ID 3875

UniProt ID P05783

Flow cytometry analysis (intracellular staining) of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-Cytokeratin 18 (C-04) FITC (red). Overlay with Isotype mouse IgG1 control (PPV-06) FITC antiobody (cat. no. 1F-457-C100, blue).

General references:

Čáp J, Čermáň J, Němeček S, Marková M, Haná V, Frysák Z: The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH-secreting pituitary adenomas. J Clin Neurosci. 2003 Jul;10(4):444-8.
PubMed

Kovařík J. et al., J. Tumor Marker Oncol. 5, 219 (1990).

Taylor-Papadimitriou J, Stampfer M, Bártek J, Lewis A, Boshell M, Lane EB, Leigh IM: Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium. J Cell Sci. 1989 Nov;94 ( Pt 3):403-13.
PubMed

Product specific references:

Vojtesek B, Staskova Z, Nenutil R, Lauerova L, Kovarik J, Rejthar A, Bartkova J, Bartek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82.
PubMed

Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5.
PubMed

Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct;7(5):495-504.
PubMed

Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24.
PubMed