Anti-Cytokeratin 18 FITC

Anti-Cytokeratin 18 FITC
Regulatory status
RUO
Antigen
Cytokeratin 18
Clone
C-04
Format
FITC
Reactivity
Mammalian
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1F-106-C100
In stock
210.00 USD

0.025 mg
1F-106-C025
In stock
105.00 USD
Variant
0.1 mg
11-261-C100
In stock
210.00 USD

0.1 mg
11-261-C100
In stock
105.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
The antibody C-04 reacts with cytokeratin 18 (45 kDa intracellular antigen), a member of intermediate filaments subfamily represented in epithelial tissues.
Application
Application details
Flow cytometry: Recommended dilution: 1-2 μg/ml. Intracellular staining.
Reactivity
Mammalian
Immunogen
Cytoskeleton preparation of epidermal carcinoma cell line A431.
Concentration
0.1 mg/ml
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS) solution containing 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
K18, CK18, CYK18, KRT18
Antigen description
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).
Entrez Gene ID 3875
UniProt ID P05783
1F-106 FC
Flow cytometry analysis (intracellular staining) of cytokeratin expression in HeLa human cervix carcinoma cell line using anti-Cytokeratin 18 (C-04) FITC (red). Overlay with Isotype mouse IgG1 control (PPV-06) FITC antiobody (cat. no. 1F-457-C100, blue).

General references:

Čáp J, Čermáň J, Němeček S, Marková M, Haná V, Frysák Z: The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH-secreting pituitary adenomas. J Clin Neurosci. 2003 Jul;10(4):444-8.
PubMed
Kovařík J. et al., J. Tumor Marker Oncol. 5, 219 (1990).
Taylor-Papadimitriou J, Stampfer M, Bártek J, Lewis A, Boshell M, Lane EB, Leigh IM: Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium. J Cell Sci. 1989 Nov;94 ( Pt 3):403-13.
PubMed

Product specific references:

Vojtesek B, Staskova Z, Nenutil R, Lauerova L, Kovarik J, Rejthar A, Bartkova J, Bartek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82.
PubMed
Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5.
PubMed
Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct;7(5):495-504.
PubMed
Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24.
PubMed
Variant
0.1 mg
1F-106-C100
In stock
210.00 USD

0.025 mg
1F-106-C025
In stock
105.00 USD
Variant
0.1 mg
11-261-C100
In stock
210.00 USD

0.1 mg
11-261-C100
In stock
105.00 USD

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