Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Cytokeratin 18 Biotin

Anti-Cytokeratin 18 Biotin

Anti-Cytokeratin 18 Biotin
Regulatory status
RUO
Antigen
Cytokeratin 18
Clone
DC-10
Format
Biotin
Reactivity
Human
Application
ELISA, IP, WB, FC (QC tested), IHC(P), ICC
Variant
0.1 mg
1B-107-C100
In stock
180.00 USD
0.025 mg
1B-107-C025
Out of stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
0.1 mg
11-261-C100
In stock
90.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody DC-10 reacts with cytokeratin 18 (45 kDa). Cytokeratins are members of intermediate filaments subfamily of intracellular proteins, represented in epithelial tissues.
Application
ELISA, IP, WB, FC (QC tested), IHC(P), ICC
Application details
Flow cytometry: Intracellular staining; recommended dilution: 2-6 μg/ml.
Reactivity
Human
Negative species
Dog, Mouse, Rat, Pig, Hamster, Sheep, Cow
Immunogen
Human breast carcinoma cell line PMC-42.
Other names
K18, CK18, CYK18, KRT18
Concentration
1 mg/ml
Preparation
The purified antibody is conjugated with biotin-LC-NHS under optimum conditions. The reagent is free of unconjugated biotin.
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1B-107 FC
Flow cytometry analysis (intracellular staining) of HeLa cells with anti-human cytokeratin 18 (DC-10) biotin antibody (red, concentration in sample 6 μg/ml) streptavidin-APC, with blank sample (black).

General references:

Čáp J, Čermáň J, Němeček S, Marková M, Haná V, Frysák Z: The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH-secreting pituitary adenomas. J Clin Neurosci. 2003 Jul;10(4):444-8.
PubMed
Kovařík J. et al., J. Tumor Marker Oncol. 5, 219 (1990).
Taylor-Papadimitriou J, Stampfer M, Bártek J, Lewis A, Boshell M, Lane EB, Leigh IM: Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium. J Cell Sci. 1989 Nov;94 ( Pt 3):403-13.
PubMed

Product specific references:

Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct;7(5):495-504.
PubMed
Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5.
PubMed
Vojtesek B, Staskova Z, Nenutil R, Lauerova L, Kovarik J, Rejthar A, Bartkova J, Bartek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82.
PubMed
Variant
0.1 mg
1B-107-C100
In stock
180.00 USD
0.025 mg
1B-107-C025
Out of stock
90.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
0.1 mg
11-261-C100
In stock
90.00 USD
Contact distributor
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