Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Cytokeratin 18 Alexa Fluor<sup>®</sup> 488

Anti-Cytokeratin 18 Alexa Fluor^® 488

Regulatory status

RUO

Antigen

Cytokeratin 18

Clone

C-04

Format

Alexa Fluor^® 488

Reactivity

Mammalian

Application

WB, FC (QC tested), ICC

Excitation laser

blue (488 nm)

Variant

0.1 mg

A4-106-C100

In stock

308.00 USD

0.025 mg

A4-106-C025

Delivery 1 week

154.00 USD

Variant

0.1 mg

A4-106-C100

In stock

308.00 USD

0.025 mg

A4-106-C025

Delivery 1 week

154.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The antibody C-04 reacts with cytokeratin 18 (45 kDa intracellular antigen), a member of intermediate filaments subfamily represented in epithelial tissues.

Application

WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 3-12 μg/ml. Intracellular staining.

Reactivity

Mammalian

Immunogen

Cytoskeleton preparation of epidermal carcinoma cell line A431.

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with Alexa Fluor^® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

K18, CK18, CYK18, KRT18

Antigen description

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. They are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families. Cytokeratin 18 belongs to type I family (acidic cytokeratins).

Entrez Gene ID 3875

UniProt ID P05783

Flow cytometry analysis (intracellular staining) of cytokeratin 18 in HeLa cells with anti-cytokeratin 18 (C-04) Alexa Fluor® 488.

General references:

Čáp J, Čermáň J, Němeček S, Marková M, Haná V, Frysák Z: The influence of treatment with somatostatin analogues on morphology, proliferative and apoptotic activity in GH-secreting pituitary adenomas. J Clin Neurosci. 2003 Jul;10(4):444-8.
PubMed

Kovařík J. et al., J. Tumor Marker Oncol. 5, 219 (1990).

Taylor-Papadimitriou J, Stampfer M, Bártek J, Lewis A, Boshell M, Lane EB, Leigh IM: Keratin expression in human mammary epithelial cells cultured from normal and malignant tissue: relation to in vivo phenotypes and influence of medium. J Cell Sci. 1989 Nov;94 ( Pt 3):403-13.
PubMed

Product specific references:

Vojtesek B, Staskova Z, Nenutil R, Lauerova L, Kovarik J, Rejthar A, Bartkova J, Bartek J: Monoclonal antibodies recognizing different epitopes of cytokeratin No.18. Folia Biol (Praha). 1989;35(6):373-82.
PubMed

Kovarik J, Rejthar A, Lauerova L, Vojtesek B, Bartkova J: Monoclonal antibodies against individual cytokeratins in the detection of metastatic spread. Int J Cancer Suppl. 1988;3:50-5.
PubMed

Lauerova L, Kovarik J, Bartek J, Rejthar A, Vojtesek B: Novel monoclonal antibodies defining epitope of human cytokeratin 18 molecule. Hybridoma. 1988 Oct;7(5):495-504.
PubMed

Bartek J, Vojtesek B, Staskova Z, Bartkova J, Kerekes Z, Rejthar A, Kovarik J: A series of 14 new monoclonal antibodies to keratins: characterization and value in diagnostic histopathology. J Pathol. 1991 Jul;164(3):215-24.
PubMed