Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-CLIC5 Purified

Anti-CLIC5 Purified

Regulatory status

RUO

Antigen

CLIC5

Clone

CLIC5-02

Format

Purified

Reactivity

Human

Application

WB, FC (QC tested)

Variant

0.1 mg

11-701-C100

In stock

203.50 USD

0.025 mg

11-701-C025

Delivery 1 week

101.75 USD

Variant

0.1 mg

11-701-C100

In stock

203.50 USD

0.025 mg

11-701-C025

Delivery 1 week

101.75 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

Mouse monoclonal antibody CLIC5-02 recognizes CLIC5a, a 32 kDa intracellular protein which associates with proteins of actin complexes. Crossreactivity with CLIC5b was not determined.

Application

WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.

Reactivity

Human

Immunogen

KLH-conjugated peptide corresponding to the amino acids 160-173 of human CLIC5a (Cys-Ile-Asp-Ala-Asn-Thr-Ser-Asp-Lys-Gly-Ser-Arg-Arg coupled with KLH).

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

MST130, DFNB102, DFNB103, MSTP130

Antigen description

CLIC5a belongs to the family of chloride intracellular channel (CLIC) proteins, all sharing a highly conserved C terminus and variable N terminus. Human CLIC5 is transcribed in two isoforms, 32 kDa CLIC5a (251 amino acids) and 49 kDa CLIC5b (410 amino acids). These proteins exist in a soluble form and their function as ion channels in vitro has not been fully confirmed in vivo. CLIC5a is a component of complexes of actin, ezrin, and several other actin-associated proteins and is important for functionality of actin-based structures.

Entrez Gene ID 53405

UniProt ID Q9NZA1

Western blotting analysis of CLIC5a in HEK293-CLIC5a transfectants (A) and HEK293 cells (B) using mouse monoclonal antibody (clone CLIC5-02).

Separation of NALM-6 cells stained using anti-CLIC5 (CLIC5-02) purified antibody (concentration in sample 5 μg/ml, GAM APC, red-filled) from NALM-6 cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

General references:

Wegner B, Al-Momany A, Kulak SC, Kozlowski K, Obeidat M, Jahroudi N, Paes J, Berryman M, Ballermann BJ: CLIC5A, a component of the ezrin-podocalyxin complex in glomeruli, is a determinant of podocyte integrity. Am J Physiol Renal Physiol. 2010 Jun;298(6):F1492-503.
PubMed