Anti-CLIC5 PE

Anti-CLIC5 PE
Regulatory status
RUO
Antigen
CLIC5
Clone
CLIC5-02
Format
PE
Reactivity
Human
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-701-C100
In stock
242.00 USD

0.025 mg
1P-701-C025
Delivery 1 week
121.00 USD
Variant
0.1 mg
1P-701-C100
In stock
242.00 USD

0.025 mg
1P-701-C025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2b kappa
Specificity
Mouse monoclonal antibody CLIC5-02 recognizes CLIC5a, a 32 kDa intracellular protein which associates with proteins of actin complexes. Crossreactivity with CLIC5b was not determined.
Application
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Reactivity
Human
Immunogen
KLH-conjugated peptide corresponding to the amino acids 160-173 of human CLIC5a (Cys-Ile-Asp-Ala-Asn-Thr-Ser-Asp-Lys-Gly-Ser-Arg-Arg coupled with KLH).
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
MST130, DFNB102, DFNB103, MSTP130
Antigen description
CLIC5a belongs to the family of chloride intracellular channel (CLIC) proteins, all sharing a highly conserved C terminus and variable N terminus. Human CLIC5 is transcribed in two isoforms, 32 kDa CLIC5a (251 amino acids) and 49 kDa CLIC5b (410 amino acids). These proteins exist in a soluble form and their function as ion channels in vitro has not been fully confirmed in vivo. CLIC5a is a component of complexes of actin, ezrin, and several other actin-associated proteins and is important for functionality of actin-based structures.
Entrez Gene ID 53405
UniProt ID Q9NZA1
1P-701 FC
Separation of CLIC5 transfected HEK-293 cells stained using anti-CLIC5 (CLIC5-02) PE antibody (concentration in sample 3 μg/ml, red-filled) from unstained CLIC5 transfected HEK-293 cells (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

General references:

Wegner B, Al-Momany A, Kulak SC, Kozlowski K, Obeidat M, Jahroudi N, Paes J, Berryman M, Ballermann BJ: CLIC5A, a component of the ezrin-podocalyxin complex in glomeruli, is a determinant of podocyte integrity. Am J Physiol Renal Physiol. 2010 Jun;298(6):F1492-503.
PubMed
Variant
0.1 mg
1P-701-C100
In stock
242.00 USD

0.025 mg
1P-701-C025
Delivery 1 week
121.00 USD
Variant
0.1 mg
1P-701-C100
In stock
242.00 USD

0.025 mg
1P-701-C025
Delivery 1 week
121.00 USD

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