Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-CLIC5 Alexa Fluor<sup>®</sup> 647

Anti-CLIC5 Alexa Fluor^® 647

Regulatory status

RUO

Antigen

CLIC5

Clone

CLIC5-02

Format

Alexa Fluor^® 647

Reactivity

Human

Application

WB, FC (QC tested)

Excitation laser

red (633 nm)

Variant

0.1 mg

A6-701-C100

In stock

248.60 USD

Variant

0.1 mg

A6-701-C100

In stock

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

Mouse monoclonal antibody CLIC5-02 recognizes CLIC5a, a 32 kDa intracellular protein which associates with proteins of actin complexes. Crossreactivity with CLIC5b was not determined.

Application

WB, FC (QC tested)

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.

Reactivity

Human

Immunogen

KLH-conjugated peptide corresponding to the amino acids 160-173 of human CLIC5a (Cys-Ile-Asp-Ala-Asn-Thr-Ser-Asp-Lys-Gly-Ser-Arg-Arg coupled with KLH).

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with Alexa Fluor^® 647 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

MST130, DFNB102, DFNB103, MSTP130

Antigen description

CLIC5a belongs to the family of chloride intracellular channel (CLIC) proteins, all sharing a highly conserved C terminus and variable N terminus. Human CLIC5 is transcribed in two isoforms, 32 kDa CLIC5a (251 amino acids) and 49 kDa CLIC5b (410 amino acids). These proteins exist in a soluble form and their function as ion channels in vitro has not been fully confirmed in vivo. CLIC5a is a component of complexes of actin, ezrin, and several other actin-associated proteins and is important for functionality of actin-based structures.

Entrez Gene ID 53405

UniProt ID Q9NZA1

Flow cytometry intracellular staining pattern of human peripheral whole blood spiked with NALM-6 cells and stained using anti-CLIC5 (CLIC5-02) Alexa Fluor® 647 antibody (concentration in sample 5 μg/ml).

Separation of NALM-6 cells (red-filled) from human neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood spiked with NALM-6 cells and stained using anti-CLIC5 (CLIC5-02) Alexa Fluor® 647 antibody (concentration in sample 5 μg/ml).

General references:

Wegner B, Al-Momany A, Kulak SC, Kozlowski K, Obeidat M, Jahroudi N, Paes J, Berryman M, Ballermann BJ: CLIC5A, a component of the ezrin-podocalyxin complex in glomeruli, is a determinant of podocyte integrity. Am J Physiol Renal Physiol. 2010 Jun;298(6):F1492-503.
PubMed