Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Clathrin Heavy Chain Purified

Anti-Clathrin Heavy Chain Purified

Regulatory status

RUO

Antigen

Clathrin Heavy Chain

Clone

BF-06

Format

Purified

Reactivity

Rat, Cow, Mouse, Human, Pig

Application

ELISA, IP, WB, FC (QC tested), ICC

Variant

0.1 mg

11-526-C100

In stock

209.00 USD

0.025 mg

11-526-C025

In stock

104.50 USD

Variant

0.1 mg

11-526-C100

In stock

209.00 USD

0.025 mg

11-526-C025

In stock

104.50 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgM

Specificity

The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa intracellular protein involved in receptor-mediated endocytosis.

Application

ELISA, IP, WB, FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 4-10 μg/ml. Intracellular staining.

Reactivity

Rat, Cow, Mouse, Human, Pig

Concentration

1 mg/ml

Preparation

Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Formulation

Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

CHC, CLHC, MRD56

Antigen description

Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.

Entrez Gene ID 1213

UniProt ID Q00610

Immunocytochemistry staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Separation of MCF-7 cells stained using anti-Clathrin Heavy Chain (BF-06) purified antibody (concentration in sample 8 μg/ml, GAM APC, red-filled) from MCF-7 cells stained using negative control (MOPC-21) purified antibody (concentration in sample 8 μg/ml, same as Clathrin Heavy Chain purified concentration, GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Immunocytochemistry staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

General references:

Wilbur JD, Hwang PK, Brodsky FM: New faces of the familiar clathrin lattice. Traffic. 2005 Apr;6(4):346-50.
PubMed

Szymkiewicz I, Shupliakov O, Dikic I: Cargo- and compartment-selective endocytic scaffold proteins. Biochem J. 2004 Oct 1;383(Pt 1):1-11.
PubMed

Smith CJ, Pearse BM: Clathrin: anatomy of a coat protein. Trends Cell Biol. 1999 Sep;9(9):335-8.
PubMed

Product specific references:

Macurek L, Zikova M, Draberova E, Draber P: Monoclonal antibody BF-06 against the heavy chain of clathrin. Folia Biol (Praha). 2003;49(6):238-40.
PubMed