Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Clathrin Heavy Chain Purified

Anti-Clathrin Heavy Chain Purified

Anti-Clathrin Heavy Chain Purified
Regulatory status
RUO
Antigen
Clathrin Heavy Chain
Clone
BF-06
Format
Purified
Reactivity
Rat, Cow, Mouse, Human, Pig
Application
ELISA, IP, WB, FC (QC tested), ICC
Variant
0.1 mg
11-526-C100
In stock
190.00 USD
0.025 mg
11-526-C025
Delivery 1 week
95.00 USD
Variant
0.1 mg
11-261-C100
In stock
190.00 USD
0.1 mg
11-261-C100
In stock
95.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgM
Specificity
The antibody BF-06 recognizes clathrin heavy chain, an ubiquitously expressed 180 kDa intracellular protein involved in receptor-mediated endocytosis.
Application
ELISA, IP, WB, FC (QC tested), ICC
Application details
Flow cytometry: Recommended dilution: 2-5 μg/ml; positive control: human blood leukocytes. Intracellular staining.
Reactivity
Rat, Cow, Mouse, Human, Pig
Concentration
1 mg/ml
Preparation
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Formulation
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
CHC, CLHC, MRD56
Antigen description
Clathrin is a submembrane protein that polymerizes into coat-like lattices, which results in membrane invagination. The basic oligomers are composed of three clathrin heavy chain (180 kDa) and three light chain (30 kDa) subunits and the process of polymerization is dynamically regulated by the light chains. Interaction of clathrin with the plasma membrane is mediated by adaptor proteins (AP1-4) specific for different cellular compartments. Another proteins, such as endophilin, epsin and amphiphysin are involved in membrane invagination and clathrin rearrangements. Finally, dynamin functions at the fission stage of clathrin-mediated endocytosis.
Entrez Gene ID 1213
UniProt ID Q00610
11-526_ICC
Immunocytochemistry staining of clathrin in human primary fibroblasts using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
11-526_ICC_2
Immunocytochemistry staining of clathrin in human HeLa cell line using anti-clathrin (BF-06; green). Actin cytoskeleton decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

General references:

Wilbur JD, Hwang PK, Brodsky FM: New faces of the familiar clathrin lattice. Traffic. 2005 Apr;6(4):346-50.
PubMed
Szymkiewicz I, Shupliakov O, Dikic I: Cargo- and compartment-selective endocytic scaffold proteins. Biochem J. 2004 Oct 1;383(Pt 1):1-11.
PubMed
Smith CJ, Pearse BM: Clathrin: anatomy of a coat protein. Trends Cell Biol. 1999 Sep;9(9):335-8.
PubMed

Product specific references:

Macurek L, Zikova M, Draberova E, Draber P: Monoclonal antibody BF-06 against the heavy chain of clathrin. Folia Biol (Praha). 2003;49(6):238-40.
PubMed
Variant
0.1 mg
11-526-C100
In stock
190.00 USD
0.025 mg
11-526-C025
Delivery 1 week
95.00 USD
Variant
0.1 mg
11-261-C100
In stock
190.00 USD
0.1 mg
11-261-C100
In stock
95.00 USD
Contact distributor
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