Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-betaIII-Tubulin FITC

Anti-betaIII-Tubulin FITC

Anti-betaIII-Tubulin FITC
Regulatory status
RUO
Antigen
betaIII-Tubulin
Clone
TU-20
Format
FITC
Reactivity
Dog, Mouse, Rat, Pig, Human
Application
FC (QC tested), ICC
Excitation laser
blue (488 nm)
Variant
0.1 mg
1F-264-C100
Out of stock
240.00 USD
0.025 mg
1F-264-C025
Out of stock
120.00 USD
Variant
0.1 mg
11-261-C100
In stock
240.00 USD
0.1 mg
11-261-C100
In stock
120.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody TU-20 recognizes C-terminal peptide sequence ESESQGPK (aa 441-448) of neuron-specific human betaIII-tubulin.
Application
FC (QC tested), ICC
Application details
Flow cytometry: Recommended dilution: 1-3 μg/ml.
Reactivity
Dog, Mouse, Rat, Pig, Human
Immunogen
Peptide (C) 441-448 coupled to maleimide-activated keyhole limpet hemocyanin via cysteine added to the N-terminus of the neuron-specific peptide.
Other names
TUBB3
Concentration
1 mg/ml
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1F-264_ICC
Immunofluorescence staining of Neuro2a mouse neuroblastoma cell line using anti-betaIII-tubulin (TU-20 FITC, green, 1 μg/ml). Nuclei were stained with DAPI (blue).

Product specific references:

Theodorou E, Dalembert G, Heffelfinger C, White E, Weissman S, Corcoran L, Snyder M: A high throughput embryonic stem cell screen identifies Oct-2 as a bifunctional regulator of neuronal differentiation. Genes Dev. 2009 Mar 1;23(5):575-88.
PubMed
Dráberová E, Sulimenko V, Vinopal S, Sulimenko T, Sládková V, D'Agostino L, Sobol M, Hozák P, Křen L, Katsetos CD, Dráber P: Differential expression of human γ-tubulin isotypes during neuronal development and oxidative stress points to a γ-tubulin-2 prosurvival function. FASEB J. 2017 May;31(5):1828-1846.
PubMed
Jirásek T, Cipro S, Musilová A, Kubecová M, Mandys V: Expression of class III beta-tubulin in colorectal carcinomas: an immunohistochemical study using TU-20 & TuJ-1 antibody. Indian J Med Res. 2009 Jan;129(1):89-94.
PubMed
Kukharskyy V, Sulimenko V, Macůrek L, Sulimenko T, Dráberová E, Dráber P: Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells. Exp Cell Res. 2004 Aug 1;298(1):218-28.
PubMed
Katsetos CD, Draberova E, Smejkalova B, Reddy G, Bertrand L, de Chadarévian J-P, Legido A, Nissanov J, Baas PW, Draber P: Class III β-Tubulin and γ-Tubulin are Co-expressed and form Complexes in Human Glioblastoma Cells. Neurochem Res (2007) 32:1387-1398.
Jirasek T, Pisarikova E, Viklicky V, Mandys V: Expression of class III beta-tubulin in malignant epithelial tumours: an immunohistochemical study using TU-20 and TuJ-1 antibodies. Folia Histochem Cytobiol. 2007;45(1):41-5.
PubMed
Peknicova J, Kubatova A, Sulimenko V, Draberova E, Viklicky V, Hozak P, Draber P: Differential subcellular distribution of tubulin epitopes in boar spermatozoa: recognition of class III beta-tubulin epitope in sperm tail. Biol Reprod. 2001 Sep;65(3):672-9.
PubMed
Draberova E, Lukas Z, Ivanyi D, Viklicky V, Draber P: Expression of class III beta-tubulin in normal and neoplastic human tissues. Histochem Cell Biol. 1998 Mar;109(3):231-9.
PubMed
Variant
0.1 mg
1F-264-C100
Out of stock
240.00 USD
0.025 mg
1F-264-C025
Out of stock
120.00 USD
Variant
0.1 mg
11-261-C100
In stock
240.00 USD
0.1 mg
11-261-C100
In stock
120.00 USD
Contact distributor
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