Anti-beta-Tubulin Purified

Anti-beta-Tubulin Purified
Regulatory status
RUO
Antigen
beta-Tubulin
Clone
TU-13
Format
Purified
Reactivity
Pig, Plants, Human, Mouse
Application
Variant
0.1 mg
11-444-C100
In stock
160.00 USD

0.025 mg
11-444-C025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD

0.1 mg
11-261-C100
In stock
80.00 USD
Product details
Description
Images
References
Isotype
Mouse IgM
Specificity
The antibody TU-13 recognizes an epitope on N-terminal structural domain of beta-tubulin in various species.
Application
Application details
Western blotting: Recommended dilution: 1-2 μg/ml.
Reactivity
Pig, Plants, Human, Mouse
Immunogen
beta-tubulin from porcine brain
Concentration
1 mg/ml
Preparation
Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).
Formulation
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TUBB
Antigen description
The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening – this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer. It is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.
Entrez Gene ID 81027
UniProt ID Q9H4B7
11-444_WB
Western blotting analysis of human beta-tubulin using mouse monoclonal antibody TU-13 on lysates of various cell lines under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-beta-tubulin monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for beta-tubulin at approximately 54 kDa.

General references:

Linhartova I, Draber P, Draberova E, Viklický V: Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins. Biochem J. 1992 Dec 15;288 ( Pt 3):919-24.
PubMed

Product specific references:

Draber P, Lagunowich LA, Draberova E, Viklicky V, Damjanov I: Heterogeneity of tubulin epitopes in mouse fetal tissues. Histochemistry. 1988;89(5):485-92.
PubMed
Draber P, Vater W, Bohm KJ, Kuklova E, Unger E: Inhibition of microtubule assembly in vitro by anti-tubulin monoclonal antibodies. FEBS Lett. 1990 Mar 26;262(2):209-11.
PubMed
Variant
0.1 mg
11-444-C100
In stock
160.00 USD

0.025 mg
11-444-C025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD

0.1 mg
11-261-C100
In stock
80.00 USD