Anti-beta-Tubulin Purified

Anti-beta-Tubulin Purified
Regulatory status
RUO
Antigen
beta-Tubulin
Clone
TU-06
Format
Purified
Reactivity
Arabidopsis, Mouse, Rat, Pig, Nicotiana, Paramecium, Chicken, Human, Fish, Tetrahymena
Application
Variant
0.1 mg
11-251-C100
In stock
160.00 USD

0.025 mg
11-251-C025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD

0.1 mg
11-261-C100
In stock
80.00 USD
Product details
Description
Images
References
Isotype
Mouse IgM
Specificity
The antibody TU-06 recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species.
Application
Application details
Immunocytochemistry: Recommended dilution: 2 μg/ml, fixed and permeabilized cells; positive control: 3T3 mouse embryonal fibroblast cell line.
Immunohistochemistry (paraffin sections): Recommended dilution: 5 μg/ml, positive tissue: heart.
Western blotting: Recommended dilution: 1 μg/ml; positive control: HPB-ALL human peripheral blood leukemia cell line, reducing conditions.
Reactivity
Arabidopsis, Mouse, Rat, Pig, Nicotiana, Paramecium, Chicken, Human, Fish, Tetrahymena
Immunogen
Beta-subunits of porcine brain tubulin.
Concentration
1 mg/ml
Preparation
Purified by precipitation and chromatography
Formulation
Tris buffered saline (TBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TUBB
Antigen description
The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening – this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer. It is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.
Entrez Gene ID 81027
UniProt ID Q9H4B7
11-251_ICC
Immunocytochemistry staining of 3T3 mouse embryonal fibroblast cell line using anti-beta-tubulin (TU-06) (detection by Goat anti-mouse IgM Cy®5). Nucleus is stained with DAPI (blue).
11-251_IHC
Immunohistochemistry staining of human heart (paraffin sections) using anti-beta tubulin (TU-06). Commercially tested by LifeSpan BioSciences.
11-251 WB
Western blotting analysis (reducing conditions) of HPB-ALL human peripheral blood leukemia cell line. Lane 1: negative control. Lane 2,3,4,5,6: immunostaining with anti-beta-tubulin (TU-06; dilution 0,5 μg/ml, 1 μg/ml, 2 μg/ml, 4 μg/ml, 5 μg/ml)

General references:

Linhartova I, Draber P, Draberova E, Viklický V: Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins. Biochem J. 1992 Dec 15;288 ( Pt 3):919-24.
PubMed

Product specific references:

Draber P, Draberova E, Linhartova I, Viklicky V: Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies. J Cell Sci. 1989 Mar;92 ( Pt 3):519-28.
PubMed
Tobita K, Liu LJ, Janczewski AM, Tinney JP, Nonemaker JM, Augustine S, Stolz DB, Shroff SG, Keller BB: Engineered early embryonic cardiac tissue retains proliferative and contractile properties of developing embryonic myocardium. Am J Physiol Heart Circ Physiol. 2006 Oct;291(4):H1829-37.
PubMed
Libusova L, Sulimenko T, Sulimenko V, Janisch R, Hozak P, Draber P: Distinct localization of a beta-tubulin epitope in the Tetrahymena thermophila and Paramecium caudatum cortex. Protoplasma. 2005 Oct;225(3-4):157-67.
PubMed
Solecki DJ, Model L, Gaetz J, Kapoor TM, Hatten ME: Par6alpha signaling controls glial-guided neuronal migration. Nat Neurosci. 2004 Nov;7(11):1195-203.
PubMed
Dryková D, Cenklová V, Sulimenko V, Volc J, Dráber P, Binarová P: Plant gamma-tubulin interacts with alphabeta-tubulin dimers and forms membrane-associated complexes. Plant Cell. 2003 Feb;15(2):465-80.
PubMed
Variant
0.1 mg
11-251-C100
In stock
160.00 USD

0.025 mg
11-251-C025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD

0.1 mg
11-261-C100
In stock
80.00 USD