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Exbio
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Research products
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Antibodies
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Cytoskeleton and ECM
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Anti-beta-Tubulin Alexa Fluor<sup>®</sup> 488
Anti-beta-Tubulin Alexa Fluor
®
488
Regulatory status
RUO
Antigen
beta-Tubulin
Clone
TU-12
Format
Alexa Fluor
®
488
Reactivity
Mouse, Rat, Pig, Human, Nicotiana, Arabidopsis
Application
FC (QC tested), ICC
Excitation laser
blue (488 nm)
Variant
0.1 mg
A4-427-C100
Ask for delivery term
HERE
242.00 USD
Variant
0.1 mg
A4-427-C100
Ask for delivery term
HERE
242.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgM
Specificity
The antibody TU-12 recognizes an epitope located within aa 345-430 of C-terminal domain of beta-tubulin in various species.
Application
FC (QC tested), ICC
Application details
Flow cytometry: Recommended dilution: 2-5 µg/ml. Intracellular staining.
Reactivity
Mouse, Rat, Pig, Human, Nicotiana, Arabidopsis
Immunogen
Porcine brain microtubule protein MTP-1.
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with Alexa Fluor
®
488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor
®
, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Other names
TUBB
Antigen description
The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening – this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The beta-tubulin (relative molecular weight around 50 kDa) is counterpart of alpha-tubulin in tubulin heterodimer. It is coded by multiple tubulin genes and it is also posttranslationally modified. Heterogeneity of subunit is concentrated in C-terminal structural domain.
Entrez Gene ID
81027
UniProt ID
Q9H4B7
Separation of HeLa cells stained using anti-beta-Tubulin (TU-12) Alexa Fluor® 488 antibody (concentration in sample 5 μg/ml, red-filled) from unstained HeLa cells in flow cytometry analysis (intracellular staining) of HeLa cell suspension.
General references:
Linhartova I, Draber P, Draberova E, Viklický V: Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins. Biochem J. 1992 Dec 15;288 ( Pt 3):919-24.
PubMed
Product specific references:
Blume Y, Yemets A, Sheremet Y, Nyporko A, Sulimenko V, Sulimenko T, Draber P: Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells. BMC Plant Biol. 2010 Feb 18;10(1):29. [Epub ahead of print]
PubMed
Smertenko A, Blume Y, Viklický V, Dráber P: Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies. Eur J Cell Biol. 1997 Feb;72(2):104-12.
PubMed
Draber P, Draberova E, Viklicky V.: Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head. Histochemistry. 1991;95(5):519-24.
PubMed
Pěknicová J, Pexidrová M, Kubátová A, Koubek P, Teplá O, Sulimenko T, Dráber P: Expression of beta-tubulin epitope in human sperm with pathological spermiogram. Fertil Steril. 2007 Oct;88(4 Suppl):1120-8.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
A4-427-C100
Ask for delivery term
HERE
242.00 USD
Variant
0.1 mg
A4-427-C100
Ask for delivery term
HERE
242.00 USD
Contact distributor
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