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Exbio
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Research products
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Antibodies
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Cytoskeleton and ECM
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Anti-beta Tubulin Alexa Fluor® 488
Anti-beta Tubulin Alexa Fluor® 488
Regulatory status
RUO
Antigen
beta-Tubulin
Clone
TU-06
Format
Alexa Fluor® 488
Reactivity
Arabidopsis, Mouse, Rat, Pig, Nicotiana, Paramecium, Chicken, Human, Fish, Tetrahymena
Application
WB, IHC(P), ICC
Excitation laser
blue (488 nm)
Variant
0.1 mg
A4-251-C100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgM
Specificity
The antibody TU-06 recognizes an epitope (aa 81-95) on phylogenetically conserved N-terminal structural domain of beta-tubulin (recognizes all beta-tubulin isoforms) in various species.
Application
WB, IHC(P), ICC
Application details
Flow cytometry: Recommended dilution: 2-5 µg/ml
Reactivity
Arabidopsis, Mouse, Rat, Pig, Nicotiana, Paramecium, Chicken, Human, Fish, Tetrahymena
Immunogen
Beta-subunits of porcine brain tubulin.
Other names
TUBB
Concentration
0.1 mg/ml
Preparation
The purified antibody is conjugated with Alexa Fluor® 488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing Tris buffered saline (TBS) solution with 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Immunocytochemistry staining of beta tubulin in HeLa cells using mouse monoclonal antibody TU-06 Alexa Fluor® 488.
General references:
Linhartova I, Draber P, Draberova E, Viklický V: Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins. Biochem J. 1992 Dec 15;288 ( Pt 3):919-24.
PubMed
Product specific references:
Draber P, Draberova E, Linhartova I, Viklicky V: Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies. J Cell Sci. 1989 Mar;92 ( Pt 3):519-28.
PubMed
Tobita K, Liu LJ, Janczewski AM, Tinney JP, Nonemaker JM, Augustine S, Stolz DB, Shroff SG, Keller BB: Engineered early embryonic cardiac tissue retains proliferative and contractile properties of developing embryonic myocardium. Am J Physiol Heart Circ Physiol. 2006 Oct;291(4):H1829-37.
PubMed
Libusova L, Sulimenko T, Sulimenko V, Janisch R, Hozak P, Draber P: Distinct localization of a beta-tubulin epitope in the Tetrahymena thermophila and Paramecium caudatum cortex. Protoplasma. 2005 Oct;225(3-4):157-67.
PubMed
Solecki DJ, Model L, Gaetz J, Kapoor TM, Hatten ME: Par6alpha signaling controls glial-guided neuronal migration. Nat Neurosci. 2004 Nov;7(11):1195-203.
PubMed
Dryková D, Cenklová V, Sulimenko V, Volc J, Dráber P, Binarová P: Plant gamma-tubulin interacts with alphabeta-tubulin dimers and forms membrane-associated complexes. Plant Cell. 2003 Feb;15(2):465-80.
PubMed
Variant
0.1 mg
A4-251-C100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Contact distributor
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