Anti-beta Tubulin Alexa Fluor® 488

Anti-beta Tubulin Alexa Fluor® 488
Regulatory status
RUO
Antigen
beta-Tubulin
Clone
TU-12
Format
Alexa Fluor® 488
Reactivity
Mouse, Rat, Pig, Human
Application
WB, FC (QC tested), ICC
Excitation laser
blue (488 nm)
Variant
0.1 mg
A4-427-C100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
Product details
Images
References
Isotype
Mouse IgM
Specificity
The antibody TU-12 recognizes an epitope located within aa 345-430 of C-terminal domain of beta-tubulin in various species.
Application
WB, FC (QC tested), ICC
Reactivity
Mouse, Rat, Pig, Human
Immunogen
Porcine brain microtubule protein MTP-1.
Other names
TUBB
Concentration
0.1 mg/ml
Preparation
The purified antibody is conjugated with Alexa Fluor® 488 under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Stabilizing Tris buffered saline (TBS) solution with 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Licence note
Alexa Fluor®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.
Licence label
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
A4-427_FC_Histogram
Separation of HeLa cells stained using anti-beta-Tubulin (TU-12) Alexa Fluor® 488 antibody (concentration in sample 5 μg/ml, red-filled) from unstained HeLa cells in flow cytometry analysis (intracellular staining) of HeLa cell suspension.

General references:

Linhartova I, Draber P, Draberova E, Viklický V: Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins. Biochem J. 1992 Dec 15;288 ( Pt 3):919-24.
PubMed

Product specific references:

Blume Y, Yemets A, Sheremet Y, Nyporko A, Sulimenko V, Sulimenko T, Draber P: Exposure of beta-tubulin regions defined by antibodies on an Arabidopsis thaliana microtubule protofilament model and in the cells. BMC Plant Biol. 2010 Feb 18;10(1):29. [Epub ahead of print]
PubMed
Smertenko A, Blume Y, Viklický V, Dráber P: Exposure of tubulin structural domains in Nicotiana tabacum microtubules probed by monoclonal antibodies. Eur J Cell Biol. 1997 Feb;72(2):104-12.
PubMed
Draber P, Draberova E, Viklicky V.: Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta-tubulin epitope in the sperm head. Histochemistry. 1991;95(5):519-24.
PubMed
Variant
0.1 mg
A4-427-C100
In stock
220.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD