Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Basic Cytokeratins Purified

Anti-Basic Cytokeratins Purified

Regulatory status

RUO

Antigen

Basic Cytokeratins

Clone

AE3

Format

Purified

Reactivity

Cow, Rabbit, Mouse, Human, Non-human primates, Chicken, Rat

Application

WB, IHC(F), IHC(P)

Variant

0.1 mg

11-769-C100

In stock

130.00 USD

0.025 mg

11-769-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-769-C100

In stock

130.00 USD

0.025 mg

11-769-C025

Delivery 1 week

65.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

Mouse monoclonal antibody AE3 recognizes all basic type cytokeratins (intracellular antigens), namely K1-K8 (58-67 kDa). This antibody reacts with all layers of epidermis (basal layer and above) and is an excellent marker for distinguishing carcinomas from non-epithelial tumours.

Application

WB, IHC(F), IHC(P)

Application details

Western blotting: Recommended dilution: 1-2 μg/ml.

Reactivity

Cow, Rabbit, Mouse, Human, Non-human primates, Chicken, Rat

Immunogen

Human epidermal keratins

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Type II cytokeratins

Antigen description

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Western blotting analysis of human basic cytokeratins using mouse monoclonal antibody AE3 on lysates of MCF-7 cell line and Molt-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-basic cytokeratins monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Specific bands were detected for basic cytokeratins at approximately 40-55 kDa.

Product specific references:

Hunt RC, Davis AA: Altered expression of keratin and vimentin in human retinal pigment epithelial cells in vivo and in vitro. J Cell Physiol. 1990 Nov;145(2):187-99.
PubMed

Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT: Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol. 1982 Nov;95(2 Pt 1):580-8.
PubMed

Zhang B, Chen Y, Han Z, Ris H, Zhai Z: The role of keratin filaments during nuclear envelope reassembly in Xenopus egg extracts. FEBS Lett. 1998 May 22;428(1-2):52-6.
PubMed

Yeh IT, Miller AR: Staining of sentinel node reticulum cells with cytokeratin antibody AE3. Arch Pathol Lab Med. 2002 Mar;126(3):248-9.
PubMed

Listrom MB, Dalton LW: Comparison of keratin monoclonal antibodies MAK-6, AE1:AE3, and CAM-5.2. Am J Clin Pathol. 1987 Sep;88(3):297-301.
PubMed