Anti-alpha-Tubulin Purified

Anti-alpha-Tubulin Purified
Regulatory status
RUO
Antigen
alpha-Tubulin
Clone
YOL1/34
Format
Purified
Reactivity
Mouse, Rat, Mammalian, Avian, Yeast, Human
Application
ELISA, WB, FC (QC tested), IHC(F), IHC(P), ICC
Variant
0.1 mg
11-886-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD
Product details
Images
References
Isotype
Rat IgG2a
Specificity
The rat monoclonal antibody YOL1/34 recognizes an epitope of alpha-tubulin localized between amino acids 414-422. It has higher affinity for fixed microtubules than for native ones.
Application
ELISA, WB, FC (QC tested), IHC(F), IHC(P), ICC
Application details
Immunocytochemistry: Recommended dilution 4-8 μg/ml.
Western blotting: Recommended dilution 1 μg/ml
Reactivity
Mouse, Rat, Mammalian, Avian, Yeast, Human
Immunogen
Yeast tubulin
Other names
TUBA
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
11-886_ICC
Immunocytochemistry staining of alpha-tubulin in K562 cells using purified rat monoclonal antibody YOL1/34 (concentration in sample 6 μg/ml, DAR FITC, left picture) vs. Hoechst 34580 nuclear staining (right picture).

Product specific references:

Badin-Larçon AC, Boscheron C, Soleilhac JM, Piel M, Mann C, Denarier E, Fourest-Lieuvin A, Lafanechère L, Bornens M, Job D: Suppression of nuclear oscillations in Saccharomyces cerevisiae expressing Glu tubulin. Proc Natl Acad Sci U S A 2004 Apr 13;101(15):5577-82
PubMed
Harris GS, Keath EJ, Medoff J: Expression of alpha- and beta-tubulin genes during dimorphic-phase transitions of Histoplasma capsulatum. Mol Cell Biol. 1989 May;9(5):2042-9
PubMed
Larcher JC, Boucher D, Lazereg S, Gros F, Denoulet P: Interaction of kinesin motor domains with alpha- and beta-tubulin subunits at a tau-independent binding site. Regulation by polyglutamylation. J Biol Chem. 1996 Sep 6;271(36):22117-24
PubMed
Breitling F, Little M: Carboxy-terminal regions on the surface of tubulin and microtubules. Epitope locations of YOL1/34, DM1A and DM1B. J Mol Biol. 1986 May 20;189(2):367-70
PubMed
Variant
0.1 mg
11-886-C100
In stock
140.00 USD
Variant
0.1 mg
11-261-C100
In stock
140.00 USD