Anti-alpha-Tubulin FITC

Anti-alpha-Tubulin FITC
Regulatory status
RUO
Antigen
alpha-Tubulin
Clone
TU-01
Format
FITC
Reactivity
Mouse, Pig, Human, Turkey, Eisenia, Paramecium, Nicotiana, Yeast, Arabidopsis
Excitation laser
blue (488 nm)
Variant
0.1 mg
1F-250-C100
In stock
242.00 USD

0.025 mg
1F-250-C025
Delivery 1 week
121.00 USD
Variant
0.1 mg
1F-250-C100
In stock
242.00 USD

0.025 mg
1F-250-C025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The antibody TU-01 recognizes a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
Application details
Flow cytometry: Recommended dilution: 1-4 μg/ml. Intracellular staining.
Reactivity
Mouse, Pig, Human, Turkey, Eisenia, Paramecium, Nicotiana, Yeast, Arabidopsis
Immunogen
Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
Concentration
1 mg/ml
Preparation
Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TUBA
Antigen description
The microtubules are intracellular dynamic polymers made up of evolutionarily conserved polymorphic alpha/beta-tubulin heterodimers and a large number of microtubule-associated proteins (MAPs). The microtubules consist of 13 protofilaments and have an outer diameter 25 nm. Microtubules have their intrinsic polarity; highly dynamic plus ends and less dynamic minus ends. Microtubules are required for vital processes in eukaryotic cells including mitosis, meiosis, maintenance of cell shape and intracellular transport. Microtubules are also necessary for movement of cells by means of flagella and cilia. In mammalian tissue culture cells microtubules have their minus ends anchored in microtubule organizing centers (MTOCs). The GTP (guanosintriphosphate) molecule is an essential for tubulin heterodimer to associate with other heterodimers to form microtubule. In vivo, microtubule dynamics vary considerably. Microtubule polymerization is reversible and a populations of microtubules in cells are on their minus ends either growing or shortening – this phenomenon is called dynamic instability of microtubules. On a practical level, microtubules can easily be stabilized by the addition of non-hydrolysable analogues of GTP (eg. GMPPCP) or more commonly by anti-cancer drugs such as Taxol. Taxol stabilizes microtubules at room temperature for many hours. Using limited proteolysis by enzymes both tubulin subunits can be divided into N-terminal and C-terminal structural domains. The alpha-tubulin (relative molecular weight around 50 kDa) is globular protein that exists in cells as part of soluble alpha/beta-tubulin dimer or it is polymerized into microtubules. In different species it is coded by multiple tubulin genes that form tubulin classes (in human 6 genes). Expressed tubulin genes are named tubulin isotypes. Some of the tubulin isotypes are expressed ubiquitously, while some have more restricted tissue expression. Alpha-tubulin is also subject of numerous post-translational modifications. Tubulin isotypes and their posttranslational modifications are responsible for multiple tubulin charge variants - tubulin isoforms. Heterogeneity of alpha-tubulin is concentrated in C-terminal structural domain.
Entrez Gene ID 7277
UniProt ID Q71U36
1F-250_ICC
Immunocytochemistry staining of alpha tubulin in HeLa cells using mouse monoclonal antibody TU-01 FITC.
1F-250_FC_Histogram
Separation of HeLa cells stained using anti-human alpha-tubulin (TU-01) FITC antibody (concentration in sample 5 μg/ml, red-filled) from HeLa cells stained using mouse IgG1 isotype control (MOPC-21) FITC antibody (concentration in sample 5 μg/ml, same as alpha-tubulin FITC concentration, black-dashed) in flow cytometry analysis (intracellular staining) of HeLa cell suspension.

Product specific references:

Kukharskyy V, Sulimenko V, Macůrek L, Sulimenko T, Dráberová E, Dráber P: Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells. Exp Cell Res. 2004 Aug 1;298(1):218-28.
PubMed
Smertenko A, Blume Y, Viklicky V, Opatrny Z, Draber P: Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells. Planta. 1997;201(3):349-58.
PubMed
Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V: Dazap2 modulates transcription driven by the Wnt effector TCF-4. Nucleic Acids Res. 2009 Mar 20. [Epub ahead of print]
PubMed
Smertenko A, Blume Y, Viklicky V, Opatrny Z, Draber P: Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells. Planta. 1997;201(3):349-58.
PubMed
Nováková M, Dráberová E, Schürmann W, Czihak G, Viklický V, Dráber P: gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies. Cell Motil Cytoskeleton. 1996;33(1):38-51.
PubMed
Linhartova I, Draber P, Draberova E, Viklicky V: Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins. Biochem J. 1992 Dec 15;288 ( Pt 3):919-24.
PubMed
Draber P, Draberova E, Viklicky V: Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta tubulin epitope in the sperm head. Histochemistry. 1991;95(5):519-24.
PubMed
Draber P, Draberova E, Linhartova I, Viklicky V: Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies. J Cell Sci. 1989 Mar;92 ( Pt 3):519-28.
PubMed
Grimm M, Breitling F, Little M: Location of the epitope for the alpha-tubulin monoclonal antibody TU-O1. Biochim Biophys Acta. 1987 Jul 24;914(1):83-8.
PubMed
Draber P, Draberova E, Zicconi D, Sellitto C, Viklicky V, Cappuccinelli P: Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin. Eur J Cell Biol. 1986 Jun;41(1):82-8.
PubMed
Viklicky V, Draber P, Hasek J, Bartek J: Production and characterization of a monoclonal antitubulin antibody. Cell Biol Int Rep. 1982 Aug;6(8):725-31.
PubMed
Pěknicová J, Pexidrová M, Kubátová A, Koubek P, Teplá O, Sulimenko T, Dráber P: Expression of beta-tubulin epitope in human sperm with pathological spermiogram. Fertil Steril. 2007 Oct;88(4 Suppl):1120-8.
PubMed
Variant
0.1 mg
1F-250-C100
In stock
242.00 USD

0.025 mg
1F-250-C025
Delivery 1 week
121.00 USD
Variant
0.1 mg
1F-250-C100
In stock
242.00 USD

0.025 mg
1F-250-C025
Delivery 1 week
121.00 USD

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