Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-alpha-Tubulin FITC

Anti-alpha-Tubulin FITC

Anti-alpha-Tubulin FITC
Regulatory status
RUO
Antigen
alpha-Tubulin
Clone
TU-01
Format
FITC
Reactivity
Mouse, Pig, Human, Turkey, Eisenia, Paramecium, Nicotiana, Yeast
Application
FC (QC tested), ICC
Excitation laser
blue (488 nm)
Variant
0.1 mg
1F-250-C100
In stock
220.00 USD
0.025 mg
1F-250-C025
Out of stock
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
0.1 mg
11-261-C100
In stock
110.00 USD
Contact distributor
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody TU-01 recognizes a defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.
Application
FC (QC tested), ICC
Application details
Flow cytometry: Intracellular staining; recommended dilution: 1-4 μg/ml.
Reactivity
Mouse, Pig, Human, Turkey, Eisenia, Paramecium, Nicotiana, Yeast
Immunogen
Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.
Other names
TUBA
Concentration
1 mg/ml
Preparation
The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
1F-250_ICC
Immunocytochemistry staining of alpha tubulin in HeLa cells using mouse monoclonal antibody TU-01 FITC.

Product specific references:

Kukharskyy V, Sulimenko V, Macůrek L, Sulimenko T, Dráberová E, Dráber P: Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells. Exp Cell Res. 2004 Aug 1;298(1):218-28.
PubMed
Smertenko A, Blume Y, Viklicky V, Opatrny Z, Draber P: Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells. Planta. 1997;201(3):349-58.
PubMed
Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V: Dazap2 modulates transcription driven by the Wnt effector TCF-4. Nucleic Acids Res. 2009 Mar 20. [Epub ahead of print]
PubMed
Smertenko A, Blume Y, Viklicky V, Opatrny Z, Draber P: Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells. Planta. 1997;201(3):349-58.
PubMed
Nováková M, Dráberová E, Schürmann W, Czihak G, Viklický V, Dráber P: gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies. Cell Motil Cytoskeleton. 1996;33(1):38-51.
PubMed
Linhartova I, Draber P, Draberova E, Viklicky V: Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins. Biochem J. 1992 Dec 15;288 ( Pt 3):919-24.
PubMed
Draber P, Draberova E, Viklicky V: Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta tubulin epitope in the sperm head. Histochemistry. 1991;95(5):519-24.
PubMed
Draber P, Draberova E, Linhartova I, Viklicky V: Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies. J Cell Sci. 1989 Mar;92 ( Pt 3):519-28.
PubMed
Grimm M, Breitling F, Little M: Location of the epitope for the alpha-tubulin monoclonal antibody TU-O1. Biochim Biophys Acta. 1987 Jul 24;914(1):83-8.
PubMed
Draber P, Draberova E, Zicconi D, Sellitto C, Viklicky V, Cappuccinelli P: Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin. Eur J Cell Biol. 1986 Jun;41(1):82-8.
PubMed
Viklicky V, Draber P, Hasek J, Bartek J: Production and characterization of a monoclonal antitubulin antibody. Cell Biol Int Rep. 1982 Aug;6(8):725-31.
PubMed
Variant
0.1 mg
1F-250-C100
In stock
220.00 USD
0.025 mg
1F-250-C025
Out of stock
110.00 USD
Variant
0.1 mg
11-261-C100
In stock
220.00 USD
0.1 mg
11-261-C100
In stock
110.00 USD
Contact distributor
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