Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-alpha-Tubulin FITC

Anti-alpha-Tubulin FITC

Regulatory status

RUO

Antigen

alpha-Tubulin

Clone

TU-01

Format

FITC

Reactivity

Mouse, Pig, Human, Turkey, Eisenia, Paramecium, Nicotiana, Yeast

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

0.1 mg

1F-250-C100

In stock

0.025 mg

1F-250-C025

Out of stock

Variant

0.1 mg

11-261-C100

In stock

0.1 mg

11-261-C100

In stock

Product details

Images

References

Isotype

Mouse IgG1

Specificity

The antibody TU-01 recognizes the defined epitope (aa 65-97) on N-terminal structural domain of alpha-tubulin.

Application

FC (QC tested)

Reactivity

Mouse, Pig, Human, Turkey, Eisenia, Paramecium, Nicotiana, Yeast

Immunogen

Fraction of tubulin purified from porcine brain by two cycles of polymerization - depolymerization.

Other names

TUBA

Concentration

1 mg/ml

Preparation

The purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

Formulation

Phosphate buffered saline (PBS) solution with 15 mM sodium azide

Storage and handling

Store in the dark at 2-8°C. Do not freeze. Avoid prolonged exposure to light. Do not use after expiration date stamped on vial label.

Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.

Immunocytochemistry staining of alpha tubulin in HeLa cells using mouse monoclonal antibody TU-01 FITC.

Product specific references:

Kukharskyy V, Sulimenko V, Macůrek L, Sulimenko T, Dráberová E, Dráber P: Complexes of gamma-tubulin with nonreceptor protein tyrosine kinases Src and Fyn in differentiating P19 embryonal carcinoma cells. Exp Cell Res. 2004 Aug 1;298(1):218-28.
PubMed

Smertenko A, Blume Y, Viklicky V, Opatrny Z, Draber P: Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells. Planta. 1997;201(3):349-58.
PubMed

Lukas J, Mazna P, Valenta T, Doubravska L, Pospichalova V, Vojtechova M, Fafilek B, Ivanek R, Plachy J, Novak J, Korinek V: Dazap2 modulates transcription driven by the Wnt effector TCF-4. Nucleic Acids Res. 2009 Mar 20. [Epub ahead of print]
PubMed

Smertenko A, Blume Y, Viklicky V, Opatrny Z, Draber P: Post-translational modifications and multiple tubulin isoforms in Nicotiana tabacum L. cells. Planta. 1997;201(3):349-58.
PubMed

Nováková M, Dráberová E, Schürmann W, Czihak G, Viklický V, Dráber P: gamma-Tubulin redistribution in taxol-treated mitotic cells probed by monoclonal antibodies. Cell Motil Cytoskeleton. 1996;33(1):38-51.
PubMed

Linhartova I, Draber P, Draberova E, Viklicky V: Immunological discrimination of beta-tubulin isoforms in developing mouse brain. Post-translational modification of non-class-III beta-tubulins. Biochem J. 1992 Dec 15;288 ( Pt 3):919-24.
PubMed

Draber P, Draberova E, Viklicky V: Immunostaining of human spermatozoa with tubulin domain-specific monoclonal antibodies. Recognition of a unique beta tubulin epitope in the sperm head. Histochemistry. 1991;95(5):519-24.
PubMed

Draber P, Draberova E, Linhartova I, Viklicky V: Differences in the exposure of C- and N-terminal tubulin domains in cytoplasmic microtubules detected with domain-specific monoclonal antibodies. J Cell Sci. 1989 Mar;92 ( Pt 3):519-28.
PubMed

Grimm M, Breitling F, Little M: Location of the epitope for the alpha-tubulin monoclonal antibody TU-O1. Biochim Biophys Acta. 1987 Jul 24;914(1):83-8.
PubMed

Draber P, Draberova E, Zicconi D, Sellitto C, Viklicky V, Cappuccinelli P: Heterogeneity of microtubules recognized by monoclonal antibodies to alpha-tubulin. Eur J Cell Biol. 1986 Jun;41(1):82-8.
PubMed

Viklicky V, Draber P, Hasek J, Bartek J: Production and characterization of a monoclonal antitubulin antibody. Cell Biol Int Rep. 1982 Aug;6(8):725-31.
PubMed

Variant

0.1 mg

1F-250-C100

In stock

0.025 mg

1F-250-C025

Out of stock

Variant

0.1 mg

11-261-C100

In stock

0.1 mg

11-261-C100

In stock

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