Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-AHNAK1 Purified

Anti-AHNAK1 Purified

Regulatory status

RUO

Antigen

AHNAK1

Clone

EM-09

Format

Purified

Reactivity

Human, Mouse

Application

IP, WB, IHC-F, ICC

Variant

0.1 mg

11-540-C100

In stock

198.00 USD

0.025 mg

11-540-C025

Delivery 1 week

99.00 USD

Variant

0.1 mg

11-540-C100

In stock

198.00 USD

0.025 mg

11-540-C025

Delivery 1 week

99.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.

Application

IP, WB, IHC-F, ICC

Application details

Immunocytochemistry: Positive control: HeLa human cervix carcinoma cell line, permeabilization is required.
Western blotting: Positive control: HeLa human cervix carcinoma cell line.

Reactivity

Human, Mouse

Immunogen

Bacterially expressed fragment of N-terminal domain of human AHNAK1.

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Desmoyokin

Antigen description

AHNAK1 (Desmoyokin) is a large (700 kDa) scaffold protein that translocates to the plasma membrane after an increas of extracellular calcium level or upon proteinkinase C activation and regulates extracellular calcium influx mediated by L-type Ca2+ channels. AHNAK1 has been implicated in diverse signal transduction proceses affecting cell differentiation and proliferation. In response to calcium-dependent intercellular contacts AHNAK1 forms multimeric complexes in the plasma membrane, connected with actin and annexin 2/S100A10 assemblies and is thus involved in organization of the plasma membrane architecture. In epithelial cells, AHNAK1 is localized in cytoplasm or is membrane-associated, but in cells of nonepithelial origin AHNAK1 is predominantly nuclear; it has a weak DNA-binding activity and associates with the DNA-ligase IV-XRCC4 complex.

Entrez Gene ID 79026

UniProt ID Q09666

Immunocytochemistry staining of AHNAK1 in human primary fibroblasts using anti-AHNAK1 (EM-09; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).

Immunocytochemistry staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).

Immunohistochemistry staining (frozen sections) of murine tongue by anti-AHNAK1 antibody (EM-09; red). Actin filaments were decorated by phalloidin (green), cell nuclei stained with DAPI (blue).

General references:

Sussman J, Stokoe D, Ossina N, Shtivelman E: Protein kinase B phosphorylates AHNAK and regulates its subcellular localization. J Cell Biol. 2001 Sep 3;154(5):1019-30.
PubMed

Gentil BJ, Delphin C, Mbele GO, Deloulme JC, Ferro M, Garin J, Baudier J: The giant protein AHNAK is a specific target for the calcium- and zinc-binding S100B protein: potential implications for Ca2+ homeostasis regulation by S100B. J Biol Chem. 2001 Jun 29;276(26):23253-61.
PubMed

Haase H, Alvarez J, Petzhold D, Doller A, Behlke J, Erdmann J, Hetzer R, Regitz-Zagrosek V, Vassort G, Morano I: Ahnak is critical for cardiac Ca(V)1.2 calcium channel function and its beta-adrenergic regulation. FASEB J. 2005 Dec;19(14):1969-77.
PubMed

Matza D, Badou A, Kobayashi KS, Goldsmith-Pestana K, Masuda Y, Komuro A, McMahon-Pratt D, Marchesi VT, Flavell RA: A scaffold protein, AHNAK1, is required for calcium signaling during T cell activation. Immunity. 2008 Jan;28(1):64-74.
PubMed

Product specific references:

Johnson EK, Zhang L, Adams ME, Phillips A, Freitas MA, Froehner SC, Green-Church KB, Montanaro F: Proteomic analysis reveals new cardiac-specific dystrophin-associated proteins. PLoS One. 2012;7(8):e43515. doi: 10.1371/journal.pone.0043515.
PubMed