Anti-AHNAK1 Purified

Anti-AHNAK1 Purified
Regulatory status
RUO
Antigen
AHNAK1
Clone
EM-09
Format
Purified
Reactivity
Human, Mouse
Application
IP, WB, IHC(F), ICC
Variant
0.1 mg
11-540-C100
In stock

0.025 mg
11-540-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock
Product details
Images
References
Isotype
Mouse IgG1
Specificity
The antibody EM-09 reacts with AHNAK1, a 700 kDa multi-functional adaptor protein expressed mainly in epithelial cell, various types of muscle cells and immune cells.
Application
IP, WB, IHC(F), ICC
Reactivity
Human, Mouse
Immunogen
Bacterially expressed fragment of N-terminal domain of human AHNAK1.
Other names
Desmoyokin
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
Unless indicated otherwise, all products are For Research Use Only and not for diagnostic use. In vivo diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s. © 2019 EXBIO Praha, a.s. All rights reserved.
11-540 ICC
Immunocytochemistry staining of AHNAK1 in human primary fibroblasts using anti-AHNAK1 (EM-09; green). Actin filaments were decorated by phalloidin (red) and cell nuclei stained with DAPI (blue).
11-540 ICC 2
Immunocytochemistry staining of AHNAK1 in HeLa cell line using anti-AHNAK1 (EM-09; red). Cell nuclei stained with DAPI (blue).

General references:

Sussman J, Stokoe D, Ossina N, Shtivelman E: Protein kinase B phosphorylates AHNAK and regulates its subcellular localization. J Cell Biol. 2001 Sep 3;154(5):1019-30.
PubMed
Gentil BJ, Delphin C, Mbele GO, Deloulme JC, Ferro M, Garin J, Baudier J: The giant protein AHNAK is a specific target for the calcium- and zinc-binding S100B protein: potential implications for Ca2+ homeostasis regulation by S100B. J Biol Chem. 2001 Jun 29;276(26):23253-61.
PubMed
Haase H, Alvarez J, Petzhold D, Doller A, Behlke J, Erdmann J, Hetzer R, Regitz-Zagrosek V, Vassort G, Morano I: Ahnak is critical for cardiac Ca(V)1.2 calcium channel function and its beta-adrenergic regulation. FASEB J. 2005 Dec;19(14):1969-77.
PubMed
Matza D, Badou A, Kobayashi KS, Goldsmith-Pestana K, Masuda Y, Komuro A, McMahon-Pratt D, Marchesi VT, Flavell RA: A scaffold protein, AHNAK1, is required for calcium signaling during T cell activation. Immunity. 2008 Jan;28(1):64-74.
PubMed

Product specific references:

Johnson EK, Zhang L, Adams ME, Phillips A, Freitas MA, Froehner SC, Green-Church KB, Montanaro F: Proteomic analysis reveals new cardiac-specific dystrophin-associated proteins. PLoS One. 2012;7(8):e43515. doi: 10.1371/journal.pone.0043515.
PubMed
Variant
0.1 mg
11-540-C100
In stock

0.025 mg
11-540-C025
Out of stock
Variant
0.1 mg
11-261-C100
In stock

0.1 mg
11-261-C100
In stock