Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Actin (muscle) Alexa Fluor<sup>®</sup> 488

Anti-Actin (muscle) Alexa Fluor^® 488

Regulatory status

RUO

Antigen

Actin (muscle)

Clone

HHF35

Format

Alexa Fluor^® 488

Reactivity

Human, Chicken, Mouse, Non-human primates, Dog, Rat, Rabbit, Cat

Application

WB (verified), IHC-F, IHC-P, ICC

Excitation laser

blue (488 nm)

Variant

0.1 mg

A4-600-C100

In stock

248.60 USD

Variant

0.1 mg

A4-600-C100

In stock

248.60 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody HHF35 recognizes muscle-specific alpha and gamma actin (42 kDa) in various species. This antibody stains skeletal, smooth and myocardial cells as well as myoepithelial cells and pericytes of small vessels. It is a widely used marker of muscle and muscle-derived cells.

Application

WB (verified), IHC-F, IHC-P, ICC

Application details

Western blotting: Recommended dilution: 2 μg/ml; positive control: murine femoral muscle, murine heart; reducing conditions.

Reactivity

Human, Chicken, Mouse, Non-human primates, Dog, Rat, Rabbit, Cat

Immunogen

SDS extracted protein fraction of human myocardium

Concentration

0.1 mg/ml

Preparation

Purified antibody is conjugated with Alexa Fluor^® 488 NHS ester under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Alexa Fluor^®, Pacific Blue™ and Pacific Orange™ are registered trademarks of Life Technologies Corporation.

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

Other names

Muscle specific actin

Antigen description

Actin is a highly conserved ubiquitous globular protein (G-actin) that polymerizes to form fibrous F-actin microfilaments. In higher eucaryotes several actin isoforms have been identified, that fall into three classes. Alpha actin is a structural component of the contractile apparatus of muscle cells or muscle-derived cells. Beta actin and gamma actin play roles in regulation of cell motility in other cell types. Specific subcellular structures such as stress fibers, focal adhesions, filopodia etc., are formed by involvement of actin cytoskeleton.

Anti-muscle actin (clone HHF35) Alexa Fluor^® 488 works in WB application under reducing conditions.

Western blotting analysis was performed on lysates prepared from murine skeletal muscle and murine heart, mixed and heated (100°C, 5 min) with reducing SDS-loading buffer. Alexa Fluor^® 488-conjugated HHF35 antibody was used in the concentration of 2 µg/ml. Muscle-specific actin was detected at ~ 42 kDa in both samples.

Product specific references:

Matsuyama A, Hisaoka M, Hashimoto H: Angioleiomyoma: a clinicopathologic and immunohistochemical reappraisal with special reference to the correlation with myopericytoma. Hum Pathol. 2007 Apr;38(4):645-51.
PubMed

Matsuo S, Susumu S, Tsutsumi R, Azuma T, Obata S, Hayashi T: Glomus tumor of the omentum: a case report. J Surg Oncol. 2007 Dec 1;96(7):633-6.
PubMed

Tsukada T, Tippens D, Gordon D, Ross R, Gown AM: HHF35, a muscle-actin-specific monoclonal antibody. I. Immunocytochemical and biochemical characterization. Am J Pathol. 1987 Jan;126(1):51-60.
PubMed

Tsukada T, McNutt MA, Ross R, Gown AM: HHF35, a muscle actin-specific monoclonal antibody. II. Reactivity in normal, reactive, and neoplastic human tissues. Am J Pathol. 1987 May;127(2):389-402.
PubMed

Schmidt RA, Cone R, Haas JE, Gown AM: Diagnosis of rhabdomyosarcomas with HHF35, a monoclonal antibody directed against muscle actins. Am J Pathol. 1988 Apr;131(1):19-28.
PubMed

Miettinen M: Antibody specific to muscle actins in the diagnosis and classification of soft tissue tumors. Am J Pathol. 1988 Jan;130(1):205-15.
PubMed

de Sousa SO, Schwarzschild M, de Araújo NS, de Araújo VC: Basal cell adenocarcinoma of the palate with squamous metaplasia. J Clin Pathol. 2000 Feb;53(2):153-6.
PubMed

Handharyani E, Ochiai K, Iwata N, Umemura T: Immunohistochemical and ultrastructural study of ito cells (fat-storing cells) in response to extrahepatic bile duct ligation in broiler chickens. J Vet Med Sci. 2001 May;63(5):547-52.
PubMed

Ijzer J, Roskams T, Molenbeek RF, Ultee T, Penning LC, Rothuizen J, van den Ingh TS: Morphological characterisation of portal myofibroblasts and hepatic stellate cells in the normal dog liver. Comp Hepatol. 2006 Nov 16;5:7.
PubMed