Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Acidic Cytokeratins Purified

Anti-Acidic Cytokeratins Purified

Anti-Acidic Cytokeratins Purified
Regulatory status
RUO
Antigen
Acidic Cytokeratins
Clone
AE1
Format
Purified
Reactivity
Rabbit, Cat, Rat, Mouse, Human, Dog, Chicken, Non-human primates
Application
FC, WB, IHC(F), IHC(P), ICC
Variant
0.1 mg
11-768-C100
In stock
130.00 USD
0.025 mg
11-768-C025
Out of stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
0.1 mg
11-261-C100
In stock
65.00 USD
Contact distributor
Product details
References
Isotype
Mouse IgG1
Specificity
Mouse monoclonal antibody AE1 recognizes acidic type cytokeratins (intracellular antigens), namely K10, 14, 15, 16, 19 (40-56 kDa). This antibody stains well the basal layer of epidermis and most epithelia.
Application
FC, WB, IHC(F), IHC(P), ICC
Application details
Flow cytometry: Intracellular staining; recommended dilution: 1-5 μg/ml.
Reactivity
Rabbit, Cat, Rat, Mouse, Human, Dog, Chicken, Non-human primates
Immunogen
Human epidermal keratins
Other names
Type I cytokeratins
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Product specific references:

Listrom MB, Dalton LW: Comparison of keratin monoclonal antibodies MAK-6, AE1:AE3, and CAM-5.2. Am J Clin Pathol. 1987 Sep;88(3):297-301.
PubMed
Hunt RC, Davis AA: Altered expression of keratin and vimentin in human retinal pigment epithelial cells in vivo and in vitro. J Cell Physiol. 1990 Nov;145(2):187-99.
PubMed
Ansai SI, Katagata Y, Yoshikawa KI, Hozumi Y, Aso K: Keratin specificity analyses of eight anti-keratin monoclonal antibodies, and their immunostaining patterns in normal skin using formalin-fixed and paraffin-embedded tissue specimens. Arch Dermatol Res. 1993;285(1-2):6-12.
PubMed
Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT: Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol. 1982 Nov;95(2 Pt 1):580-8.
PubMed
Variant
0.1 mg
11-768-C100
In stock
130.00 USD
0.025 mg
11-768-C025
Out of stock
65.00 USD
Variant
0.1 mg
11-261-C100
In stock
130.00 USD
0.1 mg
11-261-C100
In stock
65.00 USD
Contact distributor
Datasheet download
SDS download
Bulk requests
Technical support for this product