Exbio — Research products — Antibodies — Cytoskeleton and ECM — Anti-Acidic Cytokeratins Purified

Anti-Acidic Cytokeratins Purified

Regulatory status

RUO

Antigen

Acidic Cytokeratins

Clone

AE1

Format

Purified

Reactivity

Rabbit, Cat, Rat, Mouse, Human, Dog, Chicken, Non-human primates

Application

FC, WB, IHC(F), IHC(P), ICC

Variant

0.1 mg

11-768-C100

In stock

130.00 USD

0.025 mg

11-768-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-768-C100

In stock

130.00 USD

0.025 mg

11-768-C025

Delivery 1 week

65.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Mouse IgG1

Specificity

Mouse monoclonal antibody AE1 recognizes acidic type cytokeratins (intracellular antigens), namely K10, 14, 15, 16, 19 (40-56 kDa). This antibody stains well the basal layer of epidermis and most epithelia.

Application

FC, WB, IHC(F), IHC(P), ICC

Application details

Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.
Western blotting: Recommended dilution: 1-2 μg/ml.

Reactivity

Rabbit, Cat, Rat, Mouse, Human, Dog, Chicken, Non-human primates

Immunogen

Human epidermal keratins

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Type I cytokeratins

Antigen description

Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. CThey are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.

Western blotting analysis of human acidic cytokeratins using mouse monoclonal antibody AE1 on lysates of MCF-7 cell line and Molt-4 cell line (cytokeratin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-acidic cytokeratins monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. Specific bands were detected for acidic cytokeratins at approximately 40-43 kDa.

Separation of CaCo-2 cells stained using anti-Acidic Cytokeratins (AE1) purified antibody (concentration in sample 3 μg/ml, GAM APC, red-filled) from CaCo-2 unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

Product specific references:

Listrom MB, Dalton LW: Comparison of keratin monoclonal antibodies MAK-6, AE1:AE3, and CAM-5.2. Am J Clin Pathol. 1987 Sep;88(3):297-301.
PubMed

Hunt RC, Davis AA: Altered expression of keratin and vimentin in human retinal pigment epithelial cells in vivo and in vitro. J Cell Physiol. 1990 Nov;145(2):187-99.
PubMed

Ansai SI, Katagata Y, Yoshikawa KI, Hozumi Y, Aso K: Keratin specificity analyses of eight anti-keratin monoclonal antibodies, and their immunostaining patterns in normal skin using formalin-fixed and paraffin-embedded tissue specimens. Arch Dermatol Res. 1993;285(1-2):6-12.
PubMed

Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT: Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol. 1982 Nov;95(2 Pt 1):580-8.
PubMed

Variant

0.1 mg

11-768-C100

In stock

130.00 USD

0.025 mg

11-768-C025

Delivery 1 week

65.00 USD

Variant

0.1 mg

11-768-C100