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Exbio
—
Research products
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Antibodies
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Cytoskeleton and ECM
—
Anti-Acidic Cytokeratins PE
Anti-Acidic Cytokeratins PE
Regulatory status
RUO
Antigen
Acidic Cytokeratins
Clone
AE1
Format
PE
Reactivity
Rabbit, Cat, Rat, Mouse, Human, Dog, Chicken, Non-human primates
Application
FC (QC tested)
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-768-C100
Ask for delivery term
HERE
220.00 USD
0.025 mg
1P-768-C025
Delivery 1 week
110.00 USD
Variant
0.1 mg
1P-768-C100
Ask for delivery term
HERE
220.00 USD
0.025 mg
1P-768-C025
Delivery 1 week
110.00 USD
Contact distributor
Product details
Description
Images
References
Isotype
Mouse IgG1
Specificity
Mouse monoclonal antibody AE1 recognizes acidic type cytokeratins (intracellular antigens), namely K10, 14, 15, 16, 19 (40-56 kDa). This antibody stains well the basal layer of epidermis and most epithelia.
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 2-5 μg/ml. Intracellular staining.
Reactivity
Rabbit, Cat, Rat, Mouse, Human, Dog, Chicken, Non-human primates
Immunogen
Human epidermal keratins
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Type I cytokeratins
Antigen description
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. CThey are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Separation of CaCo-2 cells stained using anti-Acidic cytokeratins (AE1) PE antibody (concentration in sample 1 μg/ml, red) from unstained CaCo-2 cells (black) in flow cytometry analysis (intracellular staining).
Product specific references:
Listrom MB, Dalton LW: Comparison of keratin monoclonal antibodies MAK-6, AE1:AE3, and CAM-5.2. Am J Clin Pathol. 1987 Sep;88(3):297-301.
PubMed
Hunt RC, Davis AA: Altered expression of keratin and vimentin in human retinal pigment epithelial cells in vivo and in vitro. J Cell Physiol. 1990 Nov;145(2):187-99.
PubMed
Ansai SI, Katagata Y, Yoshikawa KI, Hozumi Y, Aso K: Keratin specificity analyses of eight anti-keratin monoclonal antibodies, and their immunostaining patterns in normal skin using formalin-fixed and paraffin-embedded tissue specimens. Arch Dermatol Res. 1993;285(1-2):6-12.
PubMed
Woodcock-Mitchell J, Eichner R, Nelson WG, Sun TT: Immunolocalization of keratin polypeptides in human epidermis using monoclonal antibodies. J Cell Biol. 1982 Nov;95(2 Pt 1):580-8.
PubMed
Variant
0.1 mg
1P-768-C100
Ask for delivery term
HERE
220.00 USD
0.025 mg
1P-768-C025
Delivery 1 week
110.00 USD
Variant
0.1 mg
1P-768-C100
Ask for delivery term
HERE
220.00 USD
0.025 mg
1P-768-C025
Delivery 1 week
110.00 USD
Contact distributor
Datasheet download
SDS download
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Related products
1P-632-C100
Mouse IgG1 Isotype Control PE
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Clone
MOPC-21
Reg. status
RUO
Application
FC, FC(IC)
0.1 mg
180.00 USD
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