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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-TNAP APC
Anti-TNAP APC
Regulatory status
RUO
Antigen
TNAP
Clone
W8B2B10
Format
APC
Reactivity
Dog, Pig, Sheep, Monkey, Goat, Human
Application
FC (QC tested)
Excitation laser
red (633 nm)
Variant
0.1 mg
1A-831-C100
Ask for delivery term
HERE
286.00 USD
Variant
0.1 mg
1A-831-C100
Ask for delivery term
HERE
286.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.
Application
FC (QC tested)
Application details
Flow cytometry: Recommended dilution: 2-5 μg/ml.
Reactivity
Dog, Pig, Sheep, Monkey, Goat, Human
Immunogen
WERI-RB-1 retinoblastoma cell line
Concentration
0.1 mg/ml
Preparation
Purified antibody is conjugated with activated allophycocyanin (APC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Tissue Non-specific Alkaline Phosphatase, MSCA-1, liver/bone/kidney alkaline phosphatase
Antigen description
Tissue non-specific alkaline phosphatase (TNAP), also known as liver/bone/kidney alkaline phosphatase, or MSCA-1 (mesenchymal stem cell antigen 1) is a selective marker for the prospective isolation of bone marrow-derived mesenchymal stem cells and mesenchymal stem-like cells. It is expressed at high levels in liver, bone, kidney, or endometrium, as well as on embryonic stem cells (ESCs). TNAP also plays a role in bone mineralization. Mutations in TNAP gene are associated with hypercalcemia and skeletal defects (hypophosphatasia).
Entrez Gene ID
249
UniProt ID
P05186
Separation of TNAP positive HeLa cells (red-filled) from TNAP negative HeLa cells (black-dashed) in flow cytometry analysis (surface staining) of HeLa cellular suspension stained using anti-TNAP (W8B2B10) APC antibody (concentration in sample 0.56 μg/ml).
Flow cytometry surface staining patterns of HeLa cells stained using anti-TNAP (W8B2B10) APC antibody (concentration in sample 0.56 μg/ml, left) or mouse IgG1 isotype control (MOPC-21) APC antibody (concentration in sample 0.56 μg/ml, same as TNAP APC concentration, right).
Product specific references:
Vogel W, Grünebach F, Messam CA, Kanz L, Brugger W, Bühring HJ: Heterogeneity among human bone marrow-derived mesenchymal stem cells and neural progenitor cells. Haematologica. 2003 Feb;88(2):126-33.
PubMed
Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W: Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007 Jun;1106:262-71.
PubMed
Sobiesiak M, Sivasubramaniyan K, Hermann C, Tan C, Orgel M, Treml S, Cerabona F, de Zwart P, Ochs U, Müller CA, Gargett CE, Kalbacher H, Bühring HJ: The mesenchymal stem cell antigen MSCA-1 is identical to tissue non-specific alkaline phosphatase. Stem Cells Dev. 2010 May;19(5):669-77.
PubMed
Rozemuller H, Prins HJ, Naaijkens B, Staal J, Bühring HJ, Martens AC: Prospective isolation of mesenchymal stem cells from multiple mammalian species using cross-reacting anti-human monoclonal antibodies. Stem Cells Dev. 2010 Dec;19(12):1911-21.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAbNaN3_SDS_v1_AU.pdf
MPAbNaN3_SDS_v1_GB.pdf
MPAbNaN3_SDS_v1_TR.pdf
MPAbNaN3_SDS_v6_AT.pdf
MPAbNaN3_SDS_v6_CH.pdf
MPAbNaN3_SDS_v6_CS.pdf
MPAbNaN3_SDS_v6_EN.pdf
MPAbNaN3_SDS_v6_ES.pdf
MPAbNaN3_SDS_v6_FR.pdf
MPAbNaN3_SDS_v6_IT.pdf
MPAbNaN3_SDS_v6_NO.pdf
MPAbNaN3_SDS_v6_PL.pdf
MPAbNaN3_SDS_v6_PT.pdf
MPAbNaN3_SDS_v6_SE.pdf
MPAbNaN3_SDS_v6_SK.pdf
MPAbNaN3_SDS_v6_SL.pdf
MPAbNaN3_SDS_v7_DE.pdf
Variant
0.1 mg
1A-831-C100
Ask for delivery term
HERE
286.00 USD
Variant
0.1 mg
1A-831-C100
Ask for delivery term
HERE
286.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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