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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-TNAP
Anti-TNAP
Regulatory status
RUO
Antigen
TNAP
Clone
W8B2B10
Format
Purified
Reactivity
Dog, Pig, Sheep, Monkey, Goat, Human
Application
FC (QC tested), ICC
Variant
0.1 mg
11-831-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Contact distributor
Product details
References
Isotype
Mouse IgG1
Specificity
The mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.
Application
FC (QC tested), ICC
Application details
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Dog, Pig, Sheep, Monkey, Goat, Human
Immunogen
WERI-RB-1 retinoblastoma cell line
Other names
Tissue Non-specific Alkaline Phosphatase, MSCA-1, liver/bone/kidney alkaline phosphatase
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Phosphate buffered saline (PBS) solution with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Product specific references:
Vogel W, Grünebach F, Messam CA, Kanz L, Brugger W, Bühring HJ: Heterogeneity among human bone marrow-derived mesenchymal stem cells and neural progenitor cells. Haematologica. 2003 Feb;88(2):126-33.
PubMed
Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W: Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007 Jun;1106:262-71.
PubMed
Sobiesiak M, Sivasubramaniyan K, Hermann C, Tan C, Orgel M, Treml S, Cerabona F, de Zwart P, Ochs U, Müller CA, Gargett CE, Kalbacher H, Bühring HJ: The mesenchymal stem cell antigen MSCA-1 is identical to tissue non-specific alkaline phosphatase. Stem Cells Dev. 2010 May;19(5):669-77.
PubMed
Rozemuller H, Prins HJ, Naaijkens B, Staal J, Bühring HJ, Martens AC: Prospective isolation of mesenchymal stem cells from multiple mammalian species using cross-reacting anti-human monoclonal antibodies. Stem Cells Dev. 2010 Dec;19(12):1911-21.
PubMed
Variant
0.1 mg
11-831-C100
In stock
180.00 USD
Variant
0.1 mg
11-261-C100
In stock
180.00 USD
Contact distributor
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