Exbio — Research products — Antibodies — CD and related antigens — Anti-TNAP

Anti-TNAP

Regulatory status

RUO

Antigen

TNAP

Clone

W8B2B10

Format

Purified

Reactivity

Dog, Pig, Sheep, Monkey, Goat, Human

Application

FC (QC tested), ICC

Variant

0.1 mg

11-831-C100

In stock

198.00 USD

Variant

0.1 mg

11-831-C100

In stock

198.00 USD

Contact distributor

Product details

Description

References

SDS download

Isotype

Mouse IgG1

Specificity

The mouse monoclonal antibody W8B2B10 recognizes TNAP (tissue non-specific alkaline phosphatase), an ectoenzyme expressed mainly on embryonic stem cells, liver, bone, and kidney cells. This antibody is suitable for characterization of bone marrow-derived MSCs, iPSCs, and ESCs.

Application

FC (QC tested), ICC

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Dog, Pig, Sheep, Monkey, Goat, Human

Immunogen

WERI-RB-1 retinoblastoma cell line

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Tissue Non-specific Alkaline Phosphatase, MSCA-1, liver/bone/kidney alkaline phosphatase

Antigen description

Tissue non-specific alkaline phosphatase (TNAP), also known as liver/bone/kidney alkaline phosphatase, or MSCA-1 (mesenchymal stem cell antigen 1) is a selective marker for the prospective isolation of bone marrow-derived mesenchymal stem cells and mesenchymal stem-like cells. It is expressed at high levels in liver, bone, kidney, or endometrium, as well as on embryonic stem cells (ESCs). TNAP also plays a role in bone mineralization. Mutations in TNAP gene are associated with hypercalcemia and skeletal defects (hypophosphatasia).

Entrez Gene ID 249

UniProt ID P05186

Product specific references:

Vogel W, Grünebach F, Messam CA, Kanz L, Brugger W, Bühring HJ: Heterogeneity among human bone marrow-derived mesenchymal stem cells and neural progenitor cells. Haematologica. 2003 Feb;88(2):126-33.
PubMed

Bühring HJ, Battula VL, Treml S, Schewe B, Kanz L, Vogel W: Novel markers for the prospective isolation of human MSC. Ann N Y Acad Sci. 2007 Jun;1106:262-71.
PubMed

Sobiesiak M, Sivasubramaniyan K, Hermann C, Tan C, Orgel M, Treml S, Cerabona F, de Zwart P, Ochs U, Müller CA, Gargett CE, Kalbacher H, Bühring HJ: The mesenchymal stem cell antigen MSCA-1 is identical to tissue non-specific alkaline phosphatase. Stem Cells Dev. 2010 May;19(5):669-77.
PubMed

Rozemuller H, Prins HJ, Naaijkens B, Staal J, Bühring HJ, Martens AC: Prospective isolation of mesenchymal stem cells from multiple mammalian species using cross-reacting anti-human monoclonal antibodies. Stem Cells Dev. 2010 Dec;19(12):1911-21.
PubMed