Exbio — Research products — Antibodies — CD and related antigens — Anti-Rt TCR gamma/delta Purified Low Endotoxin

Anti-Rt TCR gamma/delta Purified Low Endotoxin

Regulatory status

RUO

Antigen

TCR gamma/delta

Clone

V65

Format

Purified Low Endotoxin

Reactivity

Rat

Application

IP, FC (QC tested), IHC(F), FUNC

Variant

0.1 mg

12-654-C100

In stock

130.00 USD

Variant

0.1 mg

12-654-C100

In stock

130.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody V65 recognizes an extracellular epitope of TCR gamma/delta, the subtype of T cell receptor expressed mainly in epithelial tissues and at the sites of infection.

Application

IP, FC (QC tested), IHC(F), FUNC

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml.
Functional application: In vitro activation and in vivo depletion of gamma/delta TCR T cells.

Reactivity

Rat

Immunogen

TCR alpha/beta-negative CD3-positive rat T cell hybridoma III.89.1.4 line

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TCRG/D

Antigen description

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

Flow cytometry surface staining pattern of rat splenocytes stained using anti-rat TCR gamma/delta (V65) purified antibody (concentration in sample 0,6 μg/ml, DAM APC).

Separation of TCR gamma/delta positive cells (red-filled) from TCR gamma/delta negative cells (black-dashed) in flow cytometry analysis (surface staining) of rat splenocytes stained using anti-rat TCR gamma/delta (V65) purified antibody (concentration in sample 0,6 μg/ml, DAM APC).

Product specific references:

De Bernardis F, Lucciarini R, Boccanera M, Amantini C, Arancia S, Morrone S, Mosca M, Cassone A, Santoni G: Phenotypic and functional characterization of vaginal dendritic cells in a rat model of Candida albicans vaginitis. Infect Immun. 2006 Jul;74(7):4282-94.
PubMed

van den Brandt J, Kwon SH, Hünig T, McPherson KG, Reichardt HM: Sustained pre-TCR expression in Notch1IC-transgenic rats impairs T cell maturation and selection. J Immunol. 2005 Jun 15;174(12):7845-52.
PubMed

Moghaddami M, Cleland LG, Mayrhofer G: MHC II+ CD45+ cells from synovium-rich tissues of normal rats: phenotype, comparison with macrophage and dendritic cell lineages and differentiation into mature dendritic cells in vitro. Int Immunol. 2005 Aug;17(8):1103-15.
PubMed

Xystrakis E, Dejean AS, Bernard I, Druet P, Liblau R, Gonzalez-Dunia D, Saoudi A: Identification of a novel natural regulatory CD8 T-cell subset and analysis of its mechanism of regulation. Blood. 2004 Nov 15;104(10):3294-301.
PubMed

Henriksson G, Helgeland L, Midtvedt T, Stierna P, Brandtzaeg P: Immune response to Mycoplasma pulmonis in nasal mucosa is modulated by the normal microbiota. Am J Respir Cell Mol Biol. 2004 Dec;31(6):657-62.
PubMed

Tam FW, Smith J, Morel D, Karkar AM, Thompson EM, Cook HT, Pusey CD: Development of scarring and renal failure in a rat model of crescentic glomerulonephritis. Nephrol Dial Transplant. 1999 Jul;14(7):1658-66.
PubMed

Hosea HJ, Rector ES, Taylor CG: Zinc-deficient rats have fewer recent thymic emigrant (CD90+) T lymphocytes in spleen and blood. J Nutr. 2003 Dec;133(12):4239-42.
PubMed

Giezeman-Smits KM, Jonges LE, Chambers WH, Brisette-Storkus CS, Van Vlierberghe RL, Van Eendenburg JD, Eggermont AM, Fleuren GJ, Kuppen PJ: Novel monoclonal antibodies against membrane structures that are preferentially expressed on IL-2-activated rat NK cells. J Leukoc Biol. 1998 Feb;63(2):209-15.
PubMed