Exbio — Research products — Antibodies — CD and related antigens — Anti-Rt TCR alpha/beta Purified Low Endotoxin

Anti-Rt TCR alpha/beta Purified Low Endotoxin

Regulatory status

RUO

Antigen

TCR alpha/beta

Clone

R73

Format

Purified Low Endotoxin

Reactivity

Non-human primates, Rat

Application

IP, WB, FC (QC tested), IHC-F, IHC-P, ICC, FUNC

Variant

0.1 mg

12-653-C100

In stock

143.00 USD

Variant

0.1 mg

12-653-C100

In stock

143.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.

Application

IP, WB, FC (QC tested), IHC-F, IHC-P, ICC, FUNC

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml.
Functional application: In vitro activation and in vivo depletion of alpha/beta TCR T cells.

Reactivity

Non-human primates, Rat

Immunogen

Rat T blasts and erythrocytes

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TCRA/B

Antigen description

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

Flow cytometry surface staining pattern of rat thymocyte suspension stained using anti-rat TCR alpha/beta (R73) purified antibody (concentration in sample 1.6 μg/ml) GAM APC.

Separation of rat TCR alpha/beta positive thymocytes (red-filled) from TCR alpha/beta negative thymocytes (black-dashed) in flow cytometry analysis (surface staining) of rat thymocyte suspension stained using anti-rat TCR alpha/beta (R73) purified antibody (concentration in sample 1.6 μg/ml) GAM APC.

Product specific references:

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Kerstan A, Hünig T: Cutting edge: distinct TCR- and CD28-derived signals regulate CD95L, Bcl-xL, and the survival of primary T cells. J Immunol. 2004 Feb 1;172(3):1341-5.
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Carlson BC, Jansson AM, Larsson A, Bucht A, Lorentzen JC: The endogenous adjuvant squalene can induce a chronic T-cell-mediated arthritis in rats. Am J Pathol. 2000 Jun;156(6):2057-65.
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Shao H, Sun SL, Kaplan HJ, Sun D: Characterization of rat CD8+ uveitogenic T cells specific for interphotoreceptor retinal-binding protein 1177-1191: J Immunol. 2004 Aug 15;173(4):2849-54.
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Li S, Thanikachalam M, Pang M, Kawaharada N, Aitouche A, Pham SM: A clinically relevant CTLA4-Ig-based regimen induces chimerism and tolerance to heart grafts. Ann Thorac Surg. 2001 Oct;72(4):1306-10.
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Müller N, van den Brandt J, Odoardi F, Tischner D, Herath J, Flügel A, Reichardt HM: A CD28 superagonistic antibody elicits 2 functionally distinct waves of T cell activation in rats. J Clin Invest. 2008 Apr;118(4):1405-16.
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Hansson AS, Heinegård D, Holmdahl R: A new animal model for relapsing polychondritis, induced by cartilage matrix protein (matrilin-1). J Clin Invest. 1999 Sep;104(5):589-98.
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Wilhelm MJ, Pratschke J, Beato F, Taal M, Kusaka M, Hancock WW, Tilney NL: Activation of the heart by donor brain death accelerates acute rejection after transplantation. Circulation. 2000 Nov 7;102(19):2426-33.
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Kreiss M, Asmuss A, Krejci K, Lindemann D, Miyoshi-Akiyama T, Uchiyama T, Rink L, Broeren CP, Herrmann T: Contrasting contributions of complementarity-determining region 2 and hypervariable region 4 of rat BV8S2+ (Vbeta8.2) TCR to the recognition of myelin basic protein and different types of bacterial superantigens. Int Immunol. 2004 May;16(5):655-63.
PubMed

Hünig T, Wallny HJ, Hartley JK, Lawetzky A, Tiefenthaler G: A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes. J Exp Med. 1989 Jan 1;169(1):73-86.
PubMed

Fuller JM, Bogdani M, Tupling TD, Jensen RA, Pefley R, Manavi S, Cort L, Blankenhorn EP, Mordes JP, Lernmark A, Kwitek AE: Genetic dissection reveals diabetes loci proximal to the gimap5 lymphopenia gene. Physiol Genomics. 2009 Jun 10;38(1):89-97.
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Ritter T, Schröder G, Risch K, Vergopoulos A, Shean MK, Kolls J, Brock J, Lehmann M, Volk HD: Ischemia/reperfusion injury-mediated down-regulation of adenovirus-mediated gene expression in a rat heart transplantation model is inhibited by co-application of a TNFRp55-Ig chimeric construct. Gene Ther. 2000 Jul;7(14):1238-43.
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Radojevic K, Arsenovic-Ranin N, Kosec D, Pesic V, Pilipovic I, Perisic M, Plecas-Solarovic B, Leposavic G: Neonatal castration affects intrathymic kinetics of T-cell differentiation and the spleen T-cell level. J Endocrinol. 2007 Mar;192(3):669-82.
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