Anti-Rt TCR alpha/beta Purified Low Endotoxin

Anti-Rt TCR alpha/beta Purified Low Endotoxin
Regulatory status
RUO
Antigen
TCR alpha/beta
Clone
R73
Format
Purified Low Endotoxin
Reactivity
Non-human primates, Rat
Variant
0.1 mg
12-653-C100
In stock
143.00 USD
Variant
0.1 mg
12-653-C100
In stock
143.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal R73 recognizes an extracellular epitope TCR alpha/beta, the dominant subtype of T cell receptor expressed in peripheral blood.
Application details
Flow cytometry: Recommended dilution: 1-4 µg/ml.
Functional application: In vitro activation and in vivo depletion of alpha/beta TCR T cells.
Reactivity
Non-human primates, Rat
Immunogen
Rat T blasts and erythrocytes
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
TCRA/B
Antigen description
The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.
12-653_FC_Profil Značení
Flow cytometry surface staining pattern of rat thymocyte suspension stained using anti-rat TCR alpha/beta (R73) purified antibody (concentration in sample 1.6 μg/ml) GAM APC.
12-653_FC_Histogram
Separation of rat TCR alpha/beta positive thymocytes (red-filled) from TCR alpha/beta negative thymocytes (black-dashed) in flow cytometry analysis (surface staining) of rat thymocyte suspension stained using anti-rat TCR alpha/beta (R73) purified antibody (concentration in sample 1.6 μg/ml) GAM APC.

Product specific references:

Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov;26(11):2582-6.
PubMed
Kerstan A, Hünig T: Cutting edge: distinct TCR- and CD28-derived signals regulate CD95L, Bcl-xL, and the survival of primary T cells. J Immunol. 2004 Feb 1;172(3):1341-5.
PubMed
Carlson BC, Jansson AM, Larsson A, Bucht A, Lorentzen JC: The endogenous adjuvant squalene can induce a chronic T-cell-mediated arthritis in rats. Am J Pathol. 2000 Jun;156(6):2057-65.
PubMed
Shao H, Sun SL, Kaplan HJ, Sun D: Characterization of rat CD8+ uveitogenic T cells specific for interphotoreceptor retinal-binding protein 1177-1191: J Immunol. 2004 Aug 15;173(4):2849-54.
PubMed
Li S, Thanikachalam M, Pang M, Kawaharada N, Aitouche A, Pham SM: A clinically relevant CTLA4-Ig-based regimen induces chimerism and tolerance to heart grafts. Ann Thorac Surg. 2001 Oct;72(4):1306-10.
PubMed
Müller N, van den Brandt J, Odoardi F, Tischner D, Herath J, Flügel A, Reichardt HM: A CD28 superagonistic antibody elicits 2 functionally distinct waves of T cell activation in rats. J Clin Invest. 2008 Apr;118(4):1405-16.
PubMed
Hansson AS, Heinegård D, Holmdahl R: A new animal model for relapsing polychondritis, induced by cartilage matrix protein (matrilin-1). J Clin Invest. 1999 Sep;104(5):589-98.
PubMed
Wilhelm MJ, Pratschke J, Beato F, Taal M, Kusaka M, Hancock WW, Tilney NL: Activation of the heart by donor brain death accelerates acute rejection after transplantation. Circulation. 2000 Nov 7;102(19):2426-33.
PubMed
Kreiss M, Asmuss A, Krejci K, Lindemann D, Miyoshi-Akiyama T, Uchiyama T, Rink L, Broeren CP, Herrmann T: Contrasting contributions of complementarity-determining region 2 and hypervariable region 4 of rat BV8S2+ (Vbeta8.2) TCR to the recognition of myelin basic protein and different types of bacterial superantigens. Int Immunol. 2004 May;16(5):655-63.
PubMed
Hünig T, Wallny HJ, Hartley JK, Lawetzky A, Tiefenthaler G: A monoclonal antibody to a constant determinant of the rat T cell antigen receptor that induces T cell activation. Differential reactivity with subsets of immature and mature T lymphocytes. J Exp Med. 1989 Jan 1;169(1):73-86.
PubMed
Fuller JM, Bogdani M, Tupling TD, Jensen RA, Pefley R, Manavi S, Cort L, Blankenhorn EP, Mordes JP, Lernmark A, Kwitek AE: Genetic dissection reveals diabetes loci proximal to the gimap5 lymphopenia gene. Physiol Genomics. 2009 Jun 10;38(1):89-97.
PubMed
Ritter T, Schröder G, Risch K, Vergopoulos A, Shean MK, Kolls J, Brock J, Lehmann M, Volk HD: Ischemia/reperfusion injury-mediated down-regulation of adenovirus-mediated gene expression in a rat heart transplantation model is inhibited by co-application of a TNFRp55-Ig chimeric construct. Gene Ther. 2000 Jul;7(14):1238-43.
PubMed
Radojevic K, Arsenovic-Ranin N, Kosec D, Pesic V, Pilipovic I, Perisic M, Plecas-Solarovic B, Leposavic G: Neonatal castration affects intrathymic kinetics of T-cell differentiation and the spleen T-cell level. J Endocrinol. 2007 Mar;192(3):669-82.
PubMed
Variant
0.1 mg
12-653-C100
In stock
143.00 USD
Variant
0.1 mg
12-653-C100
In stock
143.00 USD

Related products

-134%
Reg. statusCE IVD
ApplicationFC
100 ml
220.00 USD
-134%
Reg. statusCE IVD
ApplicationFC
100 ml
66.00 USD
-134%
Reg. statusRUO
ApplicationFC
100 ml
55.00 USD