Anti-Rt PLSCR1 Ascites

Anti-Rt PLSCR1 Ascites
Regulatory status
RUO
Antigen
PLSCR1
Clone
13A6 [TEC-23]
Format
Ascites
Reactivity
Rat
Variant
0.1 ml
21-495-R100
In stock
160.00 USD

0.025 ml
21-495-R025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD

0.1 mg
11-261-C100
In stock
80.00 USD
Product details
Description
Images
References
Isotype
Mouse IgG2a
Specificity
The antibody 13A6 [TEC-23] binds to an intracellular epitope of rat Phospholipid Scramblase 1 (PLSCR1), an 37-49 kDa protein, accelerating bidirectional movement of plasma membrane phospholipids under conditions of elevated calcium.
Application details
Western blotting: Recommended dilution: 1 μg/ml, positive control: RBL (rat basophilic leukemia) cell line; both reducing and non-reducing conditions.
Flow cytometry: Intracellular staining.
Reactivity
Rat
Immunogen
Pooled lipid raft fraction isolated from RBL-2H3 cells
Preparation
Ascitic fluid is diluted with storage buffer to final antibody concentration 5 mg/ml.
Formulation
Undiluted ascitic fuild with 15 mM sodium azide
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on the label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
PL scramblase 1, phospholipid scramblase 1
Antigen description
PLSCR1 (phospholipid scramblase 1) is a multiply palmitoylated endofacial plasma membrane protein containing several SH3 and WW domain binding motives. In the plasma membrane, PLSCR1 plays a role in transbilayer lipid redistributions and signal transduction. Nonpalmitoylated PLSCR1, however, is able to be transported into the nucleus and bind DNA. PLSCR1 potentiates the antiviral activity of interferon and its expression is highly induced by interferons and growth factors.
Entrez Gene ID 117540
UniProt ID P58195
21-495 WB
Detection of PLSCR1 in rat basophilic leukemia (RBL) cell line lysate (A) and in PLSCR1 immunoprecipitate from RBL lysate (B). The antibody 13A6 (Tec-23) was used both for immunoprecipitation and immunodetection.
21-495_ICC
Immunofluorescence staining of PLSCR1 in rat basophilic leukemia (RBL) cell line using 13A6 (Tec-23) antibody.

General references:

Pastorelli C, Veiga J, Charles N, Voignier E, Moussu H, Monteiro RC, Benhamou M: IgE receptor type I-dependent tyrosine phosphorylation of phospholipid scramblase. J Biol Chem. 2001 Jun 8;276(23):20407-12. Epub 2001 Mar 20.
PubMed
Silverman RH, Halloum A, Zhou A, Dong B, Al-Zoghaibi F, Kushner D, Zhou Q, Zhao J, Wiedmer T, Sims PJ: Suppression of ovarian carcinoma cell growth in vivo by the interferon-inducible plasma membrane protein, phospholipid scramblase 1. Cancer Res. 2002 Jan 15;62(2):397-402.
PubMed
Dong B, Zhou Q, Zhao J, Zhou A, Harty RN, Bose S, Banerjee A, Slee R, Guenther J, Williams BR, Wiedmer T, Sims PJ, Silverman RH: Phospholipid scramblase 1 potentiates the antiviral activity of interferon. J Virol. 2004 Sep;78(17):8983-93.
PubMed

Product specific references:

Smrž D, Lebduška P, Dráberová L, Korb J, Dráber P: Engagement of phospholipid scramblase 1 in activated cells: implication for phosphatidylserine externalization and exocytosis. J Biol Chem. 2008 Apr 18;283(16):10904-18.
PubMed
Variant
0.1 ml
21-495-R100
In stock
160.00 USD

0.025 ml
21-495-R025
Delivery 1 week
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
160.00 USD

0.1 mg
11-261-C100
In stock
80.00 USD