Anti-Rt CD8b Purified Low Endotoxin

Anti-Rt CD8b Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD8b
Clone
341
Format
Purified Low Endotoxin
Reactivity
Rat
Variant
0.1 mg
12-656-C100
In stock
143.00 USD
Variant
0.1 mg
12-656-C100
In stock
143.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.
Application details
Flow cytometry: Recommended dilution: 1-4 µg/ml.
Functional application: In vitro stimulation, in vivo depletion.
Reactivity
Rat
Immunogen
CD8 positive Wistar rat splenic T cell hybridomas
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Ly-3, Ly-C, Lyt-3
Antigen description
The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Entrez Gene ID 926
UniProt ID P10966
12-656_FC_Profil Značení
Flow cytometry surface staining pattern of rat splenocyte suspension stained using anti-rat CD8b (341) purified antibody (concentration in sample 1 μg/ml, GAM APC).
12-656_FC_Histogram
Separation of murine CD8b positive cells (red-filled) from CD8b negative cells (black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension stained using anti-rat CD8b (341) purified antibody (concentration in sample 1 μg/ml, GAM APC).

Product specific references:

Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur J Immunol. 1992 Nov;22(11):2841-8.
PubMed
Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov;26(11):2582-6.
PubMed
Nohara O, Kulka M, Déry RE, Wills FL, Hirji NS, Gilchrist M, Befus AD: Regulation of CD8 expression in mast cells by exogenous or endogenous nitric oxide. J Immunol. 2001 Nov 15;167(10):5935-9.
PubMed
Ellerman KE, Like AA: Islet cell membrane antigens activate diabetogenic CD4+ T-cells in the BB/Wor rat. Diabetes. 1999 May;48(5):975-82.
PubMed
Lin TJ, Hirji N, Nohara O, Stenton GR, Gilchrist M, Befus AD: Mast cells express novel CD8 molecules that selectively modulate mediator secretion. J Immunol. 1998 Dec 1;161(11):6265-72.
PubMed
Hirji N, Lin TJ, Bissonnette E, Belosevic M, Befus AD: Mechanisms of macrophage stimulation through CD8: macrophage CD8alpha and CD8beta induce nitric oxide production and associated killing of the parasite Leishmania major. J Immunol. 1998 Jun 15;160(12):6004-11.
PubMed
Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J Immunol. 1997 Feb 15;158(4):1833-40.
PubMed
Kühnlein P, Park JH, Herrmann T, Elbe A, Hünig T: Identification and characterization of rat gamma/delta T lymphocytes in peripheral lymphoid organs, small intestine, and skin with a monoclonal antibody to a constant determinant of the gamma/delta T cell receptor. J Immunol. 1994 Aug 1;153(3):979-86.
PubMed
Mabarrack NH, Turner NL, Mayrhofer G: Recent thymic origin, differentiation, and turnover of regulatory T cells. J Leukoc Biol. 2008 Nov;84(5):1287-97.
PubMed
Variant
0.1 mg
12-656-C100
In stock
143.00 USD
Variant
0.1 mg
12-656-C100
In stock
143.00 USD

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