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Exbio
—
Research products
—
Antibodies
—
CD and related antigens
—
Anti-Rt CD8a Purified Low Endotoxin
Anti-Rt CD8a Purified Low Endotoxin
Regulatory status
RUO
Antigen
CD8a
Clone
OX-8
Format
Purified Low Endotoxin
Reactivity
Rat
Application
IP, WB, FC (QC tested), IHC(F), IHC(P), FUNC
Variant
0.1 mg
12-678-C100
Ask for delivery term
HERE
130.00 USD
Variant
0.1 mg
12-678-C100
Ask for delivery term
HERE
130.00 USD
Contact distributor
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG1 kappa
Specificity
The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal extracellular domain of rat CD8a (32-34 kDa; alpha chain of the CD8 antigen).
Application
IP, WB, FC (QC tested), IHC(F), IHC(P), FUNC
Application details
Flow cytometry: Recommended dilution: 1-4 µg/ml.
Functional application: Blocking
Reactivity
Rat
Immunogen
High Mw glycoproteins from rat thymocytes
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4
Storage and handling
Store at 2-8°C. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
Ly-2, Ly-B, Ly-35, Lyt-2
Antigen description
The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Entrez Gene ID
24930
UniProt ID
P07725
Flow cytometry surface staining pattern of rat thymocyte suspension stained using anti-rat CD8a (OX-8) purified antibody (concentration in sample 0.32 μg/ml) GAM APC.
Separation of rat CD8a positive thymocytes (red-filled) from CD8a negative thymocytes (black-dashed) in flow cytometry analysis (surface staining) of rat thymocyte suspension stained using anti-rat CD8a (OX-8) purified antibody (concentration in sample 0.32 μg/ml) GAM APC.
Product specific references:
Pino SC, O'Sullivan-Murphy B, Lidstone EA, Yang C, Lipson KL, Jurczyk A, diIorio P, Brehm MA, Mordes JP, Greiner DL, Rossini AA, Bortell R: CHOP mediates endoplasmic reticulum stress-induced apoptosis in Gimap5-deficient T cells. PLoS One. 2009;4(5):e5468.
PubMed
Katsumata Y, Harigai M, Sugiura T, Kawamoto M, Kawaguchi Y, Matsumoto Y, Kohyama K, Soejima M, Kamatani N, Hara M: Attenuation of experimental autoimmune myositis by blocking ICOS-ICOS ligand interaction. J Immunol. 2007 Sep 15;179(6):3772-9.
PubMed
Abe Y, Urakami H, Ostanin D, Zibari G, Hayashida T, Kitagawa Y, Grisham MB: Induction of Foxp3-expressing regulatory T-cells by donor blood transfusion is required for tolerance to rat liver allografts. PLoS One. 2009 Nov 23;4(11):e7840.
PubMed
Ishida S, Usui T, Yamashiro K, Kaji Y, Amano S, Ogura Y, Hida T, Oguchi Y, Ambati J, Miller JW, Gragoudas ES, Ng YS, D'Amore PA, Shima DT, Adamis AP: VEGF164-mediated inflammation is required for pathological, but not physiological, ischemia-induced retinal neovascularization. J Exp Med. 2003 Aug 4;198(3):483-9.
PubMed
Mitnacht R, Bischof A, Torres-Nagel N, Hünig T: Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats. J Immunol. 1998 Jan 15;160(2):700-7.
PubMed
Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J Immunol. 1997 Feb 15;158(4):1833-40.
PubMed
Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur J Immunol. 1992 Nov;22(11):2841-8.
PubMed
Barclay AN: The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 1981 Apr;42(4):593-600.
PubMed
Further SDS language mutations available for download below. Please contact us with request for additional languages on info@exbio.cz
MPAb_SDS_v1_GB.pdf
MPAb_SDS_v1_TR.pdf
MPAb_SDS_v6_AT.pdf
MPAb_SDS_v6_CH.pdf
MPAb_SDS_v6_CS.pdf
MPAb_SDS_v6_EN.pdf
MPAb_SDS_v6_ES.pdf
MPAb_SDS_v6_FR.pdf
MPAb_SDS_v6_IT.pdf
MPAb_SDS_v6_NO.pdf
MPAb_SDS_v6_PL.pdf
MPAb_SDS_v6_PT.pdf
MPAb_SDS_v6_SE.pdf
MPAb_SDS_v6_SK.pdf
MPAb_SDS_v7_DE.pdf
Variant
0.1 mg
12-678-C100
Ask for delivery term
HERE
130.00 USD
Variant
0.1 mg
12-678-C100
Ask for delivery term
HERE
130.00 USD
Contact distributor
Datasheet download
Bulk requests
Technical support for this product
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