Exbio — Research products — Antibodies — CD and related antigens — Anti-Rt CD8a Purified Low Endotoxin

Anti-Rt CD8a Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD8a

Clone

OX-8

Format

Purified Low Endotoxin

Reactivity

Rat

Application

IP, WB, FC (QC tested), IHC(F), IHC(P), FUNC

Variant

0.1 mg

12-678-C100

Ask for delivery term HERE

130.00 USD

Variant

0.1 mg

12-678-C100

Ask for delivery term HERE

130.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody OX-8 recognizes the hinge-like membrane-proximal extracellular domain of rat CD8a (32-34 kDa; alpha chain of the CD8 antigen).

Application

IP, WB, FC (QC tested), IHC(F), IHC(P), FUNC

Application details

Flow cytometry: Recommended dilution: 1-4 µg/ml.
Functional application: Blocking

Reactivity

Rat

Immunogen

High Mw glycoproteins from rat thymocytes

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Ly-2, Ly-B, Ly-35, Lyt-2

Antigen description

The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

Entrez Gene ID 24930

UniProt ID P07725

Flow cytometry surface staining pattern of rat thymocyte suspension stained using anti-rat CD8a (OX-8) purified antibody (concentration in sample 0.32 μg/ml) GAM APC.

Separation of rat CD8a positive thymocytes (red-filled) from CD8a negative thymocytes (black-dashed) in flow cytometry analysis (surface staining) of rat thymocyte suspension stained using anti-rat CD8a (OX-8) purified antibody (concentration in sample 0.32 μg/ml) GAM APC.

Product specific references:

Pino SC, O'Sullivan-Murphy B, Lidstone EA, Yang C, Lipson KL, Jurczyk A, diIorio P, Brehm MA, Mordes JP, Greiner DL, Rossini AA, Bortell R: CHOP mediates endoplasmic reticulum stress-induced apoptosis in Gimap5-deficient T cells. PLoS One. 2009;4(5):e5468.
PubMed

Katsumata Y, Harigai M, Sugiura T, Kawamoto M, Kawaguchi Y, Matsumoto Y, Kohyama K, Soejima M, Kamatani N, Hara M: Attenuation of experimental autoimmune myositis by blocking ICOS-ICOS ligand interaction. J Immunol. 2007 Sep 15;179(6):3772-9.
PubMed

Abe Y, Urakami H, Ostanin D, Zibari G, Hayashida T, Kitagawa Y, Grisham MB: Induction of Foxp3-expressing regulatory T-cells by donor blood transfusion is required for tolerance to rat liver allografts. PLoS One. 2009 Nov 23;4(11):e7840.
PubMed

Ishida S, Usui T, Yamashiro K, Kaji Y, Amano S, Ogura Y, Hida T, Oguchi Y, Ambati J, Miller JW, Gragoudas ES, Ng YS, D'Amore PA, Shima DT, Adamis AP: VEGF164-mediated inflammation is required for pathological, but not physiological, ischemia-induced retinal neovascularization. J Exp Med. 2003 Aug 4;198(3):483-9.
PubMed

Mitnacht R, Bischof A, Torres-Nagel N, Hünig T: Opposite CD4/CD8 lineage decisions of CD4+8+ mouse and rat thymocytes to equivalent triggering signals: correlation with thymic expression of a truncated CD8 alpha chain in mice but not rats. J Immunol. 1998 Jan 15;160(2):700-7.
PubMed

Hirji N, Lin TJ, Befus AD: A novel CD8 molecule expressed by alveolar and peritoneal macrophages stimulates nitric oxide production. J Immunol. 1997 Feb 15;158(4):1833-40.
PubMed

Torres-Nagel N, Kraus E, Brown MH, Tiefenthaler G, Mitnacht R, Williams AF, Hünig T: Differential thymus dependence of rat CD8 isoform expression. Eur J Immunol. 1992 Nov;22(11):2841-8.
PubMed

Barclay AN: The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues. Immunology. 1981 Apr;42(4):593-600.
PubMed