Anti-Rt CD4 PE

Anti-Rt CD4 PE
Regulatory status
RUO
Antigen
CD4
Clone
OX-35
Format
PE
Reactivity
Rat
Application
Excitation laser
blue (488 nm)
Variant
0.1 mg
1P-679-C100
In stock
242.00 USD

0.025 mg
1P-679-C025
Delivery 1 week
121.00 USD
Variant
0.1 mg
1P-679-C100
In stock
242.00 USD

0.025 mg
1P-679-C025
Delivery 1 week
121.00 USD
Product details
Description
Images
References
SDS download
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody OX-35 reacts with an extracellular epitope of rat CD4 transmembrane glycoprotein (55 kDa).
Application
Application details
Flow cytometry: Recommended dilution: 1-5 μg/ml.
Reactivity
Rat
Immunogen
MLR generated rat Th cells
Concentration
0.5 mg/ml
Preparation
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Formulation
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Storage and handling
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.
Other names
T4/Leu-3, L3T4
Antigen description
CD4 (T4) is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1); HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1); IL-16 (binds to CD4 domain 3), human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin. Intracellular ligands: p56LckCD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus; CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).
Entrez Gene ID 24932
UniProt ID P05540
1P-679_FC_Profil Značení
Flow cytometry surface staining pattern of rat splenocyte suspension stained using anti-rat CD4 (OX-35) PE antibody (concentration in sample 5 μg/ml).
1P-679_FC_Histogram
Separation of rat CD4 positive cells (red-filled) from rat CD4 negative cells (black-dashed) in flow cytometry analysis (surface staining) of rat splenocyte suspension stained using anti-rat CD4 (OX-35) PE antibody (concentration in sample 5 μg/ml).

Product specific references:

Monzon-Casanova E, Steiniger B, Schweigle S, Clemen H, Zdzieblo D, Starick L, Müller I, Wang CR, Rhost S, Cardell S, Pyz E, Herrmann T: CD1d expression in paneth cells and rat exocrine pancreas revealed by novel monoclonal antibodies which differentially affect NKT cell activation. PLoS One. 2010 Sep 30;5(9). pii: e13089.
PubMed
Baba T, Ishizu A, Iwasaki S, Suzuki A, Tomaru U, Ikeda H, Yoshiki T, Kasahara M: CD4+/CD8+ macrophages infiltrating at inflammatory sites: a population of monocytes/macrophages with a cytotoxic phenotype. Blood. 2006 Mar 1;107(5):2004-12.
PubMed
Baba T, Iwasaki S, Maruoka T, Suzuki A, Tomaru U, Ikeda H, Yoshiki T, Kasahara M, Ishizu A: Rat CD4+CD8+ macrophages kill tumor cells through an NKG2D- and granzyme/perforin-dependent mechanism. J Immunol. 2008 Mar 1;180(5):2999-3006.
PubMed
Viel EC, Lemarié CA, Benkirane K, Paradis P, Schiffrin EL: Immune regulation and vascular inflammation in genetic hypertension. Am J Physiol Heart Circ Physiol. 2010 Mar;298(3):H938-44.
PubMed
Ramiro-Puig E, Pérez-Cano FJ, Ramos-Romero S, Pérez-Berezo T, Castellote C, Permanyer J, Franch A, Izquierdo-Pulido M, Castell M: Intestinal immune system of young rats influenced by cocoa-enriched diet. J Nutr Biochem. 2008 Aug;19(8):555-65.
PubMed
Gelderman KA, Hultqvist M, Holmberg J, Olofsson P, Holmdahl R: T cell surface redox levels determine T cell reactivity and arthritis susceptibility. Proc Natl Acad Sci U S A. 2006 Aug 22;103(34):12831-6.
PubMed
Hishikari K, Suzuki J, Ogawa M, Isobe K, Takahashi T, Onishi M, Takayama K, Isobe M: Pharmacological activation of the prostaglandin E2 receptor EP4 improves cardiac function after myocardial ischaemia/reperfusion injury. Cardiovasc Res. 2009 Jan 1;81(1):123-32.
PubMed
Yan Y, Devos T, Yu L, Xia G, Rutgeerts O, Goebels J, Segers C, Lin Y, Vandeputte M, Waer M: Pathogenesis of autoimmunity after xenogeneic thymus transplantation. J Immunol. 2003 Jun 15;170(12):5936-46.
PubMed
Salomon I, Netzer N, Wildbaum G, Schif-Zuck S, Maor G, Karin N: Targeting the function of IFN-gamma-inducible protein 10 suppresses ongoing adjuvant arthritis. J Immunol. 2002 Sep 1;169(5):2685-93.
PubMed
Tsuji H, Kawaguchi S, Wada T, Nagoya S, Inobe M, Yamashita T, Ishii S, Uede T: Adenovirus-mediated in vivo B7-1 gene transfer induces anti-tumor immunity against pre-established primary tumor and pulmonary metastasis of rat osteosarcoma. Cancer Gene Ther. 2002 Sep;9(9):747-55.
PubMed
Dragun D, Lukitsch I, Tullius SG, Qun Y, Park JK, Schneider W, Luft FC, Haller H: Inhibition of intercellular adhesion molecule-1 with antisense deoxynucleotides prolongs renal isograft survival in the rat. Kidney Int. 1998 Dec;54(6):2113-22.
PubMed
Variant
0.1 mg
1P-679-C100
In stock
242.00 USD

0.025 mg
1P-679-C025
Delivery 1 week
121.00 USD
Variant
0.1 mg
1P-679-C100
In stock
242.00 USD

0.025 mg
1P-679-C025
Delivery 1 week
121.00 USD

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