Exbio — Research products — Antibodies — CD and related antigens — Anti-Rt CD28 Purified Low Endotoxin

Anti-Rt CD28 Purified Low Endotoxin

Regulatory status

RUO

Antigen

CD28

Clone

JJ319

Format

Purified Low Endotoxin

Reactivity

Rat

Application

IP, FC (QC tested), FUNC

Variant

0.1 mg

12-655-C100

Ask for delivery term HERE

130.00 USD

Variant

0.1 mg

12-655-C100

Ask for delivery term HERE

130.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody JJ319 reacts with an extracellular epitope of CD28, a disulfide-linked homodimeric type I glycoprotein (monomer of Mw 44 kDa) which is a critical costimulatory receptor of T cells.

Application

IP, FC (QC tested), FUNC

Application details

Flow cytometry: Recommended dilution: 2-5 µg/ml.
Functional application: Costimulation.

Reactivity

Rat

Immunogen

Mouse A20J B lymphoma cells transfected with rat CD28

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Tp44

Antigen description

CD28 is the critical T cell costimulatory receptor which provides to the cell the important second activation signal by binding CD80 and CD86 that are expressed by antigen presenting cells. Besides its costimulation role CD28 functions in preventing T cells from anergic hyporesponsive state or from undergoing premature apoptotic cell death. CD28 is also expressed on human fetal NK cells and some NK cell lines, whereas on murine NK cells the CD28 expression is much broader.

Entrez Gene ID 25660

UniProt ID P31042

Flow cytometry surface staining pattern of rat splenocytes suspension stained using anti-rat CD28 (JJ319) purified antibody (concentration in sample 4 μg/ml) GAM APC.

Separation of rat CD28 positive splenocytes (red-filled) from CD28 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of rat splenocytes suspension stained using anti-rat CD28 (JJ319) purified antibody (concentration in sample 4 μg/ml) GAM APC.

Product specific references:

Haspot F, Villemain F, Laflamme G, Coulon F, Olive D, Tiollier J, Soulillou JP, Vanhove B: Differential effect of CD28 versus B7 blockade on direct pathway of allorecognition and self-restricted responses. Blood. 2002 Mar 15;99(6):2228-34.
PubMed

Laskowski IA, Pratschke J, Wilhelm MJ, Dong VM, Beato F, Taal M, Gasser M, Hancock WW, Sayegh MH, Tilney NL: Anti-CD28 monoclonal antibody therapy prevents chronic rejection of renal allografts in rats. J Am Soc Nephrol. 2002 Feb;13(2):519-27.
PubMed

van den Brandt J, Wang D, Reichardt HM: Resistance of single-positive thymocytes to glucocorticoid-induced apoptosis is mediated by CD28 signaling. Mol Endocrinol. 2004 Mar;18(3):687-95.
PubMed

Kerstan A, Armbruster N, Leverkus M, Hünig T: Cyclosporin A abolishes CD28-mediated resistance to CD95-induced apoptosis via superinduction of caspase-3. J Immunol. 2006 Dec 1;177(11):7689-97.
PubMed

Guillonneau C, Séveno C, Dugast AS, Li XL, Renaudin K, Haspot F, Usal C, Veziers J, Anegon I, Vanhove B: Anti-CD28 antibodies modify regulatory mechanisms and reinforce tolerance in CD40Ig-treated heart allograft recipients. J Immunol. 2007 Dec 15;179(12):8164-71.
PubMed

Dengler TJ, Szabo G, Sido B, Nottmeyer W, Zimmerman R, Vahl CF, Hünig T, Meuer SC: Prolonged allograft survival but no tolerance induction by modulating CD28 antibody JJ319 after high-responder rat heart transplantation. Transplantation. 1999 Feb 15;67(3):392-8.
PubMed

Lühder F, Huang Y, Dennehy KM, Guntermann C, Müller I, Winkler E, Kerkau T, Ikemizu S, Davis SJ, Hanke T, Hünig T: Topological requirements and signaling properties of T cell-activating, anti-CD28 antibody superagonists. J Exp Med. 2003 Apr 21;197(8):955-66.
PubMed

Tacke M, Hanke G, Hanke T, Hünig T: CD28-mediated induction of proliferation in resting T cells in vitro and in vivo without engagement of the T cell receptor: evidence for functionally distinct forms of CD28. Eur J Immunol. 1997 Jan;27(1):239-47.
PubMed

Robinson LE, Clandinin MT, Field CJ: R3230AC rat mammary tumor and dietary long-chain (n-3) fatty acids change immune cell composition and function during mitogen activation. J Nutr. 2001 Jul;131(7):2021-7.
PubMed