Exbio — Research products — Antibodies — CD and related antigens — Anti-Rt CD161 Purified

Anti-Rt CD161 Purified

Regulatory status

RUO

Antigen

CD161

Clone

10/78

Format

Purified

Reactivity

Rat

Application

IP, RIA, WB, FC (QC tested), IHC-F

Variant

0.1 mg

11-657-C100

Ask for delivery term HERE

113.30 USD

Variant

0.1 mg

11-657-C100

Ask for delivery term HERE

113.30 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG1 kappa

Specificity

The mouse monoclonal antibody 10/78 recognizes CD161, an approximately 30 kDa type II transmembrane C-type lectin receptor, expressed on the plasma membrane of NK cells, dendritic cells, activated monocytes and a subset of T cells as a disulphide-linked homodimer. A common extracellular epitope on rat CD161a and b isoforms is detected.

Application

IP, RIA, WB, FC (QC tested), IHC-F

Application details

Flow cytometry: Recommended dilution: 1-4 μg/ml.

Reactivity

Rat

Immunogen

Splenic cells purified from the LEW rat

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

NKRP1A, Klrb

Antigen description

CD161, also known as Nkrp1 (natural killer receptor protein 1) or Klrb1 (killer cell lectin-like receptor subfamily b member 1), is a disulphide-linked homodimeric receptor, which is involved in regulation of NK cell and NKT cell function. It is expressed on rat NK cells, subset of T cells, dendritic cells, and activated monocytes. Although human CD161 is expressed as one isoform, the rat CD161 has three isoforms, referred to as CD161a, b, and c. These proteins contain C-terminal C-type lectin extracellular domain, a transmembrane domain, and N-terminal intracellular domain, which contains ITIM motif, such as CD161b, and displays inhibitory function, or does not contain ITIM motif, thus also not the inhibitory function, such as CD161a.

Entrez Gene ID 689817

UniProt ID Q0ZUP0

Flow cytometry surface staining pattern of rat splenocyte suspension stained using anti-rat CD161 (10/78) purified antibody (concentration in sample 0,5 μg/ml) GAM APC.

Separation of rat CD161 positive splenocytes (red-filled) from CD161 negative splenocytes (black-dashed) in flow cytometry analysis (surface staining) of rat splenocyte suspension stained using anti-rat CD161 (10/78) purified antibody (concentration in sample 0,5 μg/ml) GAM APC.

Product specific references:

Tliba O, Chauvin A, Le Vern Y, Boulard C, Sbille P: Evaluation of the hepatic NK cell response during the early phase of Fasciola hepatica infection in rats. Vet Res. 2002 May-Jun;33(3):327-32.
PubMed

Sedgwick JD, Ford AL, Foulcher E, Airriess R: Central nervous system microglial cell activation and proliferation follows direct interaction with tissue-infiltrating T cell blasts. J Immunol. 1998 Jun 1;160(11):5320-30.
PubMed

Kraus E, Lambracht D, Wonigeit K, Hünig T: Negative regulation of rat natural killer cell activity by major histocompatibility complex class I recognition. Eur J Immunol. 1996 Nov;26(11):2582-6.
PubMed

May E, Dorris ML, Satumtira N, Iqbal I, Rehman MI, Lightfoot E, Taurog JD: CD8 alpha beta T cells are not essential to the pathogenesis of arthritis or colitis in HLA-B27 transgenic rats. J Immunol. 2003 Jan 15;170(2):1099-105.
PubMed

Teshima R, Nakamura R, Nakamura R, Hachisuka A, Sawada JI, Shibutani M: Effects of exposure to decabromodiphenyl ether on the development of the immune system in rats. J Health Sci 2008;54(4):382-389

Chang CJ, Tai KF, Roffler S, Hwang LH: The immunization site of cytokine-secreting tumor cell vaccines influences the trafficking of tumor-specific T lymphocytes and antitumor efficacy against regional tumors. J Immunol. 2004 Nov 15;173(10):6025-32.
PubMed

Stephens LA, Barclay AN, Mason D: Phenotypic characterization of regulatory CD4+CD25+ T cells in rats. Int Immunol. 2004 Feb;16(2):365-75.
PubMed