Exbio — Research products — Antibodies — CD and related antigens — Anti-Perforin PE

Anti-Perforin PE

Regulatory status

RUO

Antigen

Perforin

Clone

dG9

Format

Reactivity

Human, Cow

Application

FC (QC tested)

Excitation laser

blue (488 nm)

Variant

100 tests

1P-765-T100

In stock

242.00 USD

25 tests

1P-765-T025

In stock

121.00 USD

Variant

100 tests

1P-765-T100

In stock

242.00 USD

25 tests

1P-765-T025

In stock

121.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Mouse IgG2b kappa

Specificity

The mouse monoclonal antibody dG9 (also known as deltaG9) recognizes perforin, a 70 kDa protein expressed in cytoplasmic granules of cytotoxic T cells and NK cells.

Application

FC (QC tested)

Application details

Flow cytometry: The reagent is designed for analysis of human blood cells using 10 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests. Intracellular staining.

Reactivity

Human, Cow

Negative species

Mouse

Immunogen

purified granules from human YT lymphoma cell line

Preparation

Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

PRF1, P1, PFP, HPLH2

Antigen description

Perforin is a 70 kDa cytolytic protein with structural and functional similarities to complement component 9 (C9). It is stored in cytoplasmic granules of cytotoxic T cells and NK cells and after its release it forms transmembrane pores in the target cells to kill it. As perforin is a key effector molecule for cell-mediated cytolysis, defects of its gene can cause severe disorders.

Entrez Gene ID 5551

UniProt ID P14222

Anti-human Perforin PE antibody (clone dG9) works in flow cytometry application.

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats and permeabilized using EXCELLYSE XPerm solution (#ED7397).
Mouse monoclonal anti-human Perforin PE antibody (clone dG9) was used in concentration 10 µg/ml in stained blood sample (2 x 10⁶ cells).

Expression profiling on peripheral blood subsets using Anti-human Perforin PE antibody (clone dG9).

Analysis of the antibody staining profile was performed on blood leukocytes isolated from buffy coats and permeabilized using EXCELLYSE XPerm buffer set (#ED7397).
HCDM CDMaps standardized procedures (Kuzilkova D et al. Front Immunol. 2022;13:827898) were used for leukocyte isolation.
Suspension of blood leukocytes isolated from buffy coats (2 x 10⁶ cells) was added to the mixture of optimized backbone antibody panels (HLDA Innate and HLDA Adaptive) and Monocyte Blocking Buffer (#ED7747), vortexed and incubated for 20 min.
Next, EXCELLYSE XPerm buffer set (#ED7397) was used for leukocytes permeabilization according to the instruction procedure.
Mouse monoclonal anti-human Perforin PE antibody (clone dG9) was used in concentration 10 µg/ml in stained blood sample (2 x 10⁶ cells).

Product specific references:

Vargas-Inchaustegui DA, Demberg T, Robert-Guroff M: A CD8α(-) subpopulation of macaque circulatory natural killer cells can mediate both antibody-dependent and antibody-independent cytotoxic activities. Immunology. 2011 Nov;134(3):326-40.
PubMed

Takeuchi M, Inoue T, Otani T, Yamasaki F, Nakamura S, Kibata M: Cell-in-cell structures formed between human cancer cell lines and the cytotoxic regulatory T-cell line HOZOT. J Mol Cell Biol. 2010 Jun;2(3):139-51.
PubMed

Qiu Y, Chen J, Liao H, Zhang Y, Wang H, Li S, Luo Y, Fang D, Li G, Zhou B, Shen L, Chen CY, Huang D, Cai J, Cao K, Jiang L, Zeng G, Chen ZW: Tim-3-expressing CD4+ and CD8+ T cells in human tuberculosis (TB) exhibit polarized effector memory phenotypes and stronger anti-TB effector functions. PLoS Pathog. 2012;8(11):e1002984.
PubMed

Hersperger AR, Makedonas G, Betts MR: Flow cytometric detection of perforin upregulation in human CD8 T cells. Cytometry A. 2008 Nov;73(11):1050-7.
PubMed

Hameed A, Olsen KJ, Cheng L, Fox WM, Hruban RH, Podack ER: Immunohistochemical identification of cytotoxic lymphocytes using human perforin monoclonal antibody. Am J Pathol. 1992 May;140(5):1025-30.
PubMed