Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms TCR beta Purified Low Endotoxin

Anti-Ms TCR beta Purified Low Endotoxin

Regulatory status

RUO

Antigen

TCR beta

Clone

H57-597

Format

Purified Low Endotoxin

Reactivity

Mouse

Application

IP, FC (QC tested), IHC(F), IHC(P), ICC, FUNC

Variant

0.1 mg

12-571-C100

Ask for delivery term HERE

88.00 USD

Variant

0.1 mg

12-571-C100

Ask for delivery term HERE

88.00 USD

Contact distributor

Product details

Description

Images

References

SDS download

Isotype

Armenian Hamster IgG2b kappa

Specificity

The Armenian hamster monoclonal antibody H57-597 reacts specifically with an extracellular epitope on beta subunit of TCR alpha/beta. This antibody does not crossreact with TCR gamma/delta type and has been used as TCR alpha/beta phenotypic marker.

Application

IP, FC (QC tested), IHC(F), IHC(P), ICC, FUNC

Application details

Functional application: in vitro T cell stimulation, in vivo T cell depletion.
Flow cytometry: Recommended dilution: 1-2 μg/ml per 1 million cells.
Immunoprecipitation: Recommended dilution: 1-2 μg / 100-500 μg of protein in 1 ml lysate.

Reactivity

Mouse

Immunogen

Affinity purified TCR from DO-11.10 T cell hybridoma

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

TCRB

Antigen description

The antigen-specific T cell receptor (TCR) is composed of either alpha and beta subunit, or gamma and delta subunit. Majority of T cells present in the blood, lymph and secondary lymphoid organs express TCR alpha/beta heterodimers, whereas the T cells expressing TCR gamma/delta heterodimers are localized mainly in epithelial tissues and at the sites of infection. The subunits of TCR heterodimers are covalently bonded and in the endoplasmic reticulum they associate with CD3 subunits to form functional TCR-CD3 complex. Lack of expression of any of the chains is sufficient to stop cell surface expression.

Entrez Gene ID 21577

Flow cytometry surface staining pattern of murine splenocytes stained using anti-mouse TCR beta (H57-597) purified antibody (concentration in sample 3 μg/ml, GaArH APC).

Separation of murine splenocytes stained using anti-mouse TCR beta (H57-597) purified antibody (concentration in sample 3 μg/ml, GaArH APC, red-filled) from murine splenocytes unstained by primary antibody (GaArH APC, black-dashed) in flow cytometry analysis (surface staining) of murine splenocyte suspension.

Product specific references:

Rozdzial MM, Kubo RT, Turner SL, Finkel TH: Developmental regulation of the TCR zeta-chain. Differential expression and tyrosine phosphorylation of the TCR zeta-chain in resting immature and mature T lymphocytes. J Immunol. 1994 Aug 15;153(4):1563-80.
PubMed

Oliver PM, Cao X, Worthen GS, Shi P, Briones N, MacLeod M, White J, Kirby P, Kappler J, Marrack P, Yang B: Ndfip1 protein promotes the function of itch ubiquitin ligase to prevent T cell activation and T helper 2 cell-mediated inflammation. Immunity. 2006 Dec;25(6):929-40.
PubMed

Kubo RT, Born W, Kappler JW, Marrack P, Pigeon M: Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors. J Immunol. 1989 Apr 15;142(8):2736-42.
PubMed

Goodman T, Lefrancois L: Intraepithelial lymphocytes. Anatomical site, not T cell receptor form, dictates phenotype and function. J Exp Med. 1989 Nov 1;170(5):1569-81.
PubMed

Gascoigne NR: Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3. J Biol Chem. 1990 Jun 5;265(16):9296-301.
PubMed

Skeen MJ, Ziegler HK: Intercellular interactions and cytokine responsiveness of peritoneal alpha/beta and gamma/delta T cells from Listeria-infected mice: synergistic effects of interleukin 1 and 7 on gamma/delta T cells. J Exp Med. 1993 Sep 1;178(3):985-96.
PubMed

Skeen MJ, Ziegler HK: Induction of murine peritoneal gamma/delta T cells and their role in resistance to bacterial infection. J Exp Med. 1993 Sep 1;178(3):971-84.
PubMed

McCormack JE, Kappler J, Marrack P: Stimulation with specific antigen can block superantigen-mediated deletion of T cells in vivo. Proc Natl Acad Sci U S A. 1994 Mar 15;91(6):2086-90.
PubMed

van der Heyde HC, Elloso MM, Chang WL, Kaplan M, Manning DD, Weidanz WP: Gamma delta T cells function in cell-mediated immunity to acute blood-stage Plasmodium chabaudi adami malaria. J Immunol. 1995 Apr 15;154(8):3985-90.
PubMed

Kishimoto H, Kubo RT, Yorifuji H, Nakayama T, Asano Y, Tada T: Physical dissociation of the TCR-CD3 complex accompanies receptor ligation. J Exp Med. 1995 Dec 1;182(6):1997-2006.
PubMed