Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms NK1.1 Purified Low Endotoxin

Anti-Ms NK1.1 Purified Low Endotoxin

Anti-Ms NK1.1 Purified Low Endotoxin
Regulatory status
RUO
Antigen
NK1.1
Clone
PK136
Format
Purified Low Endotoxin
Reactivity
Mouse
Application
IP, FC (QC tested), IHC(F), FUNC
Variant
0.1 mg
12-758-C100
In stock
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
80.00 USD
Contact distributor
Product details
References
Isotype
Mouse IgG2a kappa
Specificity
The mouse monoclonal antibody PK136 recognizes an extracellular epitope of NK1.1 antigen (NK cell marker) expressed by some mouse strains (e.g. C57BL/6, NZB, CE, C58, Ma/My), whereas other strains (e.g. BALB/c, AKR, CBA, C3H) do not express this antigen. This antibody detects a conformational epitope on Nkrp1c and Nkrp1b gene products.
Application
IP, FC (QC tested), IHC(F), FUNC
Application details
Functional application: In vivo depletion of NK cells and NK-T cells.
Flow cytometry: Recommended dilution: 1-4 µg/ml
Reactivity
Mouse
Immunogen
NK1-positive murine splenic and bone marrow cells
Other names
Ly59, CD161, Klrb1c, Ly55c
Concentration
1 mg/ml
Preparation
Purified by protein-A affinity chromatography
Formulation
Low endotoxin azide free phosphate buffered saline (PBS) solution, 0.2 μm filter sterilized. Endotoxin level is less than 0.01 EU/μg of the protein, as determined by the LAL test.
Storage and handling
Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Exbio licence note
The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Product specific references:

Carlyle JR, Mesci A, Ljutic B, Belanger S, Tai LH, Rousselle E, Troke AD, Proteau MF, Makrigiannis AP: Molecular and genetic basis for strain-dependent NK1.1 alloreactivity of mouse NK cells. J Immunol. 2006 Jun 15;176(12):7511-24.
PubMed
GeurtsvanKessel CH, Bergen IM, Muskens F, Boon L, Hoogsteden HC, Osterhaus AD, Rimmelzwaan GF, Lambrecht BN: Both conventional and interferon killer dendritic cells have antigen-presenting capacity during influenza virus infection. PLoS One. 2009 Sep 28;4(9):e7187.
PubMed
Símová J, Bubeník J, Bieblová J, Jandlová T: The role of NK1.1+ cells in the protection against MHC class I+ HPV16-associated tumours. Folia Biol (Praha). 2004;50(6):200-2.
PubMed
Carroll JL, Nielsen LL, Pruett SB, Mathis JM: The role of natural killer cells in adenovirus-mediated p53 gene therapy. Mol Cancer Ther. 2001 Nov;1(1):49-60.
PubMed
Joncker NT, Shifrin N, Delebecque F, Raulet DH: Mature natural killer cells reset their responsiveness when exposed to an altered MHC environment. J Exp Med. 2010 Sep 27;207(10):2065-72.
PubMed
Wang M, Ellison CA, Gartner JG, HayGlass KT: Natural killer cell depletion fails to influence initial CD4 T cell commitment in vivo in exogenous antigen-stimulated cytokine and antibody responses. J Immunol. 1998 Feb 1;160(3):1098-105.
PubMed
Takahashi S, Kawamura T, Kanda Y, Taniguchi T, Nishizawa T, Iiai T, Hatakeyama K, Abo T: Activation of CD1d-independent NK1.1+ T cells in the large intestine by Lactobacilli. Immunol Lett. 2006 Jan 15;102(1):74-8.
PubMed
Fang M, Lanier LL, Sigal LJ: A role for NKG2D in NK cell-mediated resistance to poxvirus disease. PLoS Pathog. 2008 Feb 8;4(2):e30.
PubMed
Kulesza J, Hoser G, Wasilewska D, Kawiak J: NK cell depletion and recovery in SCID mice treated with anti-NK1.1 antibody. Folia Histochem Cytobiol. 2006;44(2):93-6.
PubMed
Laffont S, Seillet C, Ortaldo J, Coudert JD, Guéry JC: Natural killer cells recruited into lymph nodes inhibit alloreactive T-cell activation through perforin-mediated killing of donor allogeneic dendritic cells. Blood. 2008 Aug 1;112(3):661-71
PubMed
Variant
0.1 mg
12-758-C100
In stock
80.00 USD
Variant
0.1 mg
11-261-C100
In stock
80.00 USD
Contact distributor
Datasheet download
SDS download
Bulk requests
Technical support for this product