Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms NK1.1 Purified Low Endotoxin

Anti-Ms NK1.1 Purified Low Endotoxin

Regulatory status

RUO

Antigen

NK1.1

Clone

PK136

Format

Purified Low Endotoxin

Reactivity

Mouse

Application

IP, FC (QC tested), IHC(F), FUNC

Variant

0.1 mg

12-758-C100

Ask for delivery term HERE

80.00 USD

Variant

0.1 mg

12-758-C100

Ask for delivery term HERE

80.00 USD

Contact distributor

Product details

Description

References

SDS download

Isotype

Mouse IgG2a kappa

Specificity

The mouse monoclonal antibody PK136 recognizes an extracellular epitope of NK1.1 antigen (NK cell marker) expressed by some mouse strains (e.g. C57BL/6, NZB, CE, C58, Ma/My), whereas other strains (e.g. BALB/c, AKR, CBA, C3H) do not express this antigen. This antibody detects a conformational epitope on Nkrp1c and Nkrp1b gene products.

Application

IP, FC (QC tested), IHC(F), FUNC

Application details

Functional application: In vivo depletion of NK cells and NK-T cells.
Flow cytometry: Recommended dilution: 1-4 µg/ml

Reactivity

Mouse

Immunogen

NK1-positive murine splenic and bone marrow cells

Concentration

1 mg/ml

Preparation

Purified by protein-A affinity chromatography.

Formulation

Phosphate buffered saline (PBS), pH 7.4

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Ly59, CD161, Klrb1c, Ly55c

Antigen description

NK1.1 / CD161bc (also known as NKRP1, Ly55c, or Ly59) is a cell surface antigen expressed on NK cells and NK-T cells of certain mouse strains, which is being used for identification or antibody-mediated depletion of these cells in the respective strains. This antigen participates in NK cell activation, including production of interferon gamma, and release of cytotoxic granules.

Entrez Gene ID 17059

UniProt ID P27814

Product specific references:

Carlyle JR, Mesci A, Ljutic B, Belanger S, Tai LH, Rousselle E, Troke AD, Proteau MF, Makrigiannis AP: Molecular and genetic basis for strain-dependent NK1.1 alloreactivity of mouse NK cells. J Immunol. 2006 Jun 15;176(12):7511-24.
PubMed

GeurtsvanKessel CH, Bergen IM, Muskens F, Boon L, Hoogsteden HC, Osterhaus AD, Rimmelzwaan GF, Lambrecht BN: Both conventional and interferon killer dendritic cells have antigen-presenting capacity during influenza virus infection. PLoS One. 2009 Sep 28;4(9):e7187.
PubMed

Símová J, Bubeník J, Bieblová J, Jandlová T: The role of NK1.1+ cells in the protection against MHC class I+ HPV16-associated tumours. Folia Biol (Praha). 2004;50(6):200-2.
PubMed

Carroll JL, Nielsen LL, Pruett SB, Mathis JM: The role of natural killer cells in adenovirus-mediated p53 gene therapy. Mol Cancer Ther. 2001 Nov;1(1):49-60.
PubMed

Joncker NT, Shifrin N, Delebecque F, Raulet DH: Mature natural killer cells reset their responsiveness when exposed to an altered MHC environment. J Exp Med. 2010 Sep 27;207(10):2065-72.
PubMed

Wang M, Ellison CA, Gartner JG, HayGlass KT: Natural killer cell depletion fails to influence initial CD4 T cell commitment in vivo in exogenous antigen-stimulated cytokine and antibody responses. J Immunol. 1998 Feb 1;160(3):1098-105.
PubMed

Takahashi S, Kawamura T, Kanda Y, Taniguchi T, Nishizawa T, Iiai T, Hatakeyama K, Abo T: Activation of CD1d-independent NK1.1+ T cells in the large intestine by Lactobacilli. Immunol Lett. 2006 Jan 15;102(1):74-8.
PubMed

Fang M, Lanier LL, Sigal LJ: A role for NKG2D in NK cell-mediated resistance to poxvirus disease. PLoS Pathog. 2008 Feb 8;4(2):e30.
PubMed

Kulesza J, Hoser G, Wasilewska D, Kawiak J: NK cell depletion and recovery in SCID mice treated with anti-NK1.1 antibody. Folia Histochem Cytobiol. 2006;44(2):93-6.
PubMed

Laffont S, Seillet C, Ortaldo J, Coudert JD, Guéry JC: Natural killer cells recruited into lymph nodes inhibit alloreactive T-cell activation through perforin-mediated killing of donor allogeneic dendritic cells. Blood. 2008 Aug 1;112(3):661-71
PubMed