Exbio — Research products — Antibodies — CD and related antigens — Anti-Ms Ly6G Biotin

Anti-Ms Ly6G Biotin

Regulatory status

RUO

Antigen

Ly6G

Clone

RB6-8C5

Format

Biotin

Reactivity

Mouse

Application

IP, WB, FC (QC tested), IHC(F), IHC(P)

Variant

0.1 mg

1B-598-C100

Ask for delivery term HERE

150.00 USD

Variant

0.1 mg

1B-598-C100

Ask for delivery term HERE

150.00 USD

Contact distributor

Product details

Description

Images

References

Isotype

Rat IgG2b

Specificity

The rat monoclonal antibody RB6-8C5 detects Ly6G component of Gr-1 antigen, a commonly used surface marker of neutrophils.

Application

IP, WB, FC (QC tested), IHC(F), IHC(P)

Application details

Flow cytometry: Recommended dilution: 2-5 μg/ml.

Reactivity

Mouse

Immunogen

Murine granulocytes

Concentration

1 mg/ml

Preparation

Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Formulation

Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Storage and handling

Store at 2-8°C. Do not freeze.

The product is intended For Research Use Only. Diagnostic or therapeutic applications are strictly forbidden. Products shall not be used for resale or transfer to third parties either as a stand-alone product or as a manufacture component of another product without written consent of EXBIO Praha, a.s. EXBIO Praha, a.s. will not be held responsible for patent infringement or any other violations of intellectual property rights that may occur with the use of the products. Orders for all products are accepted subject to the Term and Conditions available at www.exbio.cz. EXBIO, EXBIO Logo, and all other trademarks are property of EXBIO Praha, a.s.

Other names

Gr1, Gr-1, Ly-6G

Antigen description

Ly6G is a component of the myeloid differentiation antigen Gr-1, together with Ly6C. Ly6G is a good marker for detection of peripheral neutrophils. Expression of Gr-1 in bone marrow correlates with granulocyte differentiation and maturation. Physiological role of Ly6G remains still unclear. Its treatment with antibodies in vivo leads to neutropenia and has inhibitory effect on local immune responses.

Entrez Gene ID 546644

UniProt ID A0A087WQF5

Flow cytometry surface staining pattern of murine peritoneal fluid cell suspension stained using anti-murine Ly6G (RB6-8C5) Biotin antibody (concentration in sample 1,0 μg/ml, Streptavidin APC).

Separation of murine myeloid Ly6G positive cells stained using anti-Ly6G (RB-8C5) Biotin antibody (concentration in sample 1,0 μg/ml, Streptavidin APC, red-filled) from murine myeloid cells unstained by primary antibody (Streptavidin APC, black-dashed) in flow cytometry analysis.

Product specific references:

Fan J, Li Y, Levy RM, Fan JJ, Hackam DJ, Vodovotz Y, Yang H, Tracey KJ, Billiar TR, Wilson MA: Hemorrhagic shock induces NAD(P)H oxidase activation in neutrophils: role of HMGB1-TLR4 signaling. J Immunol. 2007 May 15;178(10):6573-80.
PubMed

Koo GC, Gan YH: The innate interferon gamma response of BALB/c and C57BL/6 mice to in vitro Burkholderia pseudomallei infection. BMC Immunol. 2006 Aug 18;7:19.
PubMed

dos Santos MS, Vaz Cardoso LP, Nascimento GR, Lino Rde S Jr, Dorta ML, de Oliveira MA, Ribeiro-Dias F: Leishmania major: recruitment of Gr-1+ cells into draining lymph nodes during infection is important for early IL-12 and IFN gamma production. Exp Parasitol. 2008 Jul;119(3):403-10.
PubMed

Leendertse M, Willems RJ, Giebelen IA, Roelofs JJ, Bonten MJ, van der Poll T: Neutrophils are essential for rapid clearance of Enterococcus faecium in mice. Infect Immun. 2009 Jan;77(1):485-91.
PubMed

Jung YW, Zindl CL, Lai JF, Weaver CT, Chaplin DD: MMP induced by Gr-1+ cells are crucial for recruitment of Th cells into the airways. Eur J Immunol. 2009 Aug;39(8):2281-92.
PubMed

Hestdal K, Ruscetti FW, Ihle JN, Jacobsen SE, Dubois CM, Kopp WC, Longo DL, Keller JR: Characterization and regulation of RB6-8C5 antigen expression on murine bone marrow cells. J Immunol. 1991 Jul 1;147(1):22-8.
PubMed

Fleming TJ, Fleming ML, Malek TR: Selective expression of Ly-6G on myeloid lineage cells in mouse bone marrow. RB6-8C5 mAb to granulocyte-differentiation antigen (Gr-1) detects members of the Ly-6 family. J Immunol. 1993 Sep 1;151(5):2399-408.
PubMed

Stoppacciaro A, Melani C, Parenza M, Mastracchio A, Bassi C, Baroni C, Parmiani G, Colombo MP: Regression of an established tumor genetically modified to release granulocyte colony-stimulating factor requires granulocyte-T cell cooperation and T cell-produced interferon gamma. J Exp Med. 1993 Jul 1;178(1):151-61.
PubMed

Brown CR, Blaho VA, Loiacono CM: Susceptibility to experimental Lyme arthritis correlates with KC and monocyte chemoattractant protein-1 production in joints and requires neutrophil recruitment via CXCR2. J Immunol. 2003 Jul 15;171(2):893-901.
PubMed

Nagendra S, Schlueter AJ: Absence of cross-reactivity between murine Ly-6C and Ly-6G. Cytometry A. 2004 Apr;58(2):195-200.
PubMed

Variant

0.1 mg

1B-598-C100

Ask for delivery term HERE

150.00 USD

Variant

0.1 mg

1B-598-C100

Ask for delivery term HERE

150.00 USD

Contact distributor